Home     About SciClips     Contact Us     Register     Login     Upload Videos    
Open Innovation Platform for Scientific Breakthroughs, Collaborations and Philanthropism
Advertise | Consultancy
  Stem Cell Research | Proteomics / Biomarkers / Metabolomics | Drug Discovery | Biofuels | Tuberculosis | Mosquito-Borne Diseases
Visit our section on
Drug Discovery Protocols: This database contains a comprehensive list of various bioprotocols that are used in biomarker discovery. These complete protocols include techniques used in molecular and protein drug discovery research. We have listed unique protocols that are not available in any other online bioprotocol database.
Animal models

Mouse Model Choroidal Neovascularization (CNV) Induction

Choroid neovascularization (CNV), a hallmark of wet AMD, is triggered by laser photocoagulation (532 nm, 200 mW, 100 ms, 75 mM) (OcuLight GL, Iridex, Mountain View, Calif.) performed on both eyes of each mouse on day 0 by a single individual masked to drug group assignment. Laser spots are applied in a standardized fashion around the optic nerve, using a slit lamp delivery system and a cover slip as a contact lens. For evaluation, the eyes are enucleated and fixed with 4% paraformaldehyde for 30 min at 4 degree C. The neurosensory retina is detached and severed from the optic nerve. The remaining RPE-choroid-sclera complex is flat mounted in Immu-Mount (Vectashield Mounting Medium, Vector) and coverslipped. Flat mounts are examined with a scanning laser confocal microscope (TCS SP, Leica, Germany). Vessels are visualized by exciting with blue argon laser. Horizontal optical sections (1 mM step) are obtained from the surface of the RPE-choroid-sclera complex. The deepest focal plane in which the surrounding choroidal vascular network connecting to the lesion can be identified is judged to be the floor of the lesion. Any vessel in the laser treated area and superficial to this reference plane is judged as CNV. Images of each section are digitally stored. The area of CNV-related fluorescence is measured by computerized image analysis using the Leica TCS SP software. The summation of whole fluorescent area in each horizontal section is used as an index for the volume of CNV. The siRNA compounds disclosed herein are tested in this animal model which shows that these siRNAs treat and/or prevent AMD and thus may be used to treat this condition.

Source: 7,872,119

Disclaimer: Sciclips does not guarantee the authenticity or validity of the protocols listed in this section. These protocols are extracted "as is" from various sources, with minor modifications. Please refer to the respective citations, which are hyperlinked, for more details. The use of cited molecules, not limited to proteins, nucleic acids, compounds etc, in the protocols that are extracted from patents may be covered under patent rights. These molecules should be used for information purpose only.
Search Biomarker Database
This feature is available only to customers who have subscribed to premium membership. If you have subscribed to our premium membership please login to access this feature. If you wish to signup for Sciclips/subscribe to our premium membership, please click here. You will need a Sciclips account to subscribe.
Services offered

SciClips Consultancy

Fostering innovation through scientific intelligence
Sciclips consultancy service team is comprised of highly qualified and experienced scientists who have several years of research experience in academic and industrial laboratories. We offer following custom services at competitive price:

Scientific and technical areas covered: Drug discovery, Stem cells, Genomics, Proteomics, Biomarkers, Clinical diagnostics, Companion diagnostics, Molecular diagnostics, Personalized medicine (theranostics), Plant and agricultural biotechnology

Patent search

: Custom intellectual property (IP) analysis; Comprehensive patent database search and evaluation

Custom data analysis and data mining

: From patents, research articles, meeting abstracts and web based information. Custom meta analysis.

Competitive intelligence analysis

: Comprehensive analysis of competitive technologies and products; Technology trend prediction; Identification of technology licensing opportunities; Acquisition target identification; Identification of collaborative technology development opportunities.

Technology development

: Identification of potential development opportunities in drug discovery assay development, cell based assay development, recombinant protein expression (in vitro and in vivo) and mass spectrometry/proteomics reagents and tools; Product development strategy.

Diagnostics and Theranostics technology development

: Diagnostic assay development; Diagnostic target identification (molecular or protein or miRNA or metabolomic biomarkers); Prognostic and drug efficacy biomarkers; Theranostics tools, biomarkers and therapeutic drug targets; Identification of emerging technologies and methods.

Custom database development

Customized bioinformatics tools

: Offered through our partners.

Proteomics and Drug discovery services (laboratory based)

: Offered through our partners.

For more information on our consultancy services please contact us at contact@sciclips.com or email us using the contact form
Receive news updates by email
If you would like to receive news updates by email, please sign up below by entering your Name and Email address and clicking Sign Up
Keywords: High Content Screening, Cell Signaling Assays, Methyltransferase Assay, Drug Profiling Service, Pre-Clinical Services, Drug discovery assays, Cell based assays, HTS assays, clinical research services, GPCR cassays, kinase assays, phosphatase assays, toxicity assays, Reporter Assays,GPCR cell-based assays, Enzyme Inhibitors, screening molecules,Screening Assays,Screening compounds, Small Molecule Screening,Small Molecule Libraries, Raman Spectroscopy,kinase assays, protease assays, caspase assay,apoptosis assays, cytochrome P450 assays, ADME/TOX, Receptor Assay, nuclear Receptor Assay,Receptor Binding Assay,Multiplex Assays, calcium assays, Ion Channel Screening, Sodium Channel Screening,HERG channel Assay,genotyping, ATP assay,Glutathione Assay, Genotoxicity assay,Flow Cytometry, Cell Proliferation Assay, Next generation sequencing, contract assay service, High Throughput Screening, Assay development, Drug Discovery Services, Drug Profiling Service, Compound Profiling, Medicinal chemistry,ion channel assays,immunogeneticity assays, angiogenesis assays, cell migration assays, primary cell assays, cAMP assay, transporter assays

SciClips LLC, 1202 Ann Street, Madison, WI 53713 USA; contact@sciclips.com
Privacy Policy All Rights Reserved 2009-2014.