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We have a unique collection of protocols from journals and patents. We have listed thousands of protocols and in most cases, the titles of protocols listed in our site are similar to the "Materials & Methods" section of the journals. Sometimes, it is not easy to categorize these protocols. We are in the process of categorizing these protocols. Meantime, we strongly recommend you to search for the protocols using the search function.
Bioprotocols > Biochemical assays

Biochemical assays Protocols

The protocols listed in this section are extracted from scientific journals or patents or laboratory websites. We have cited only the journals that are freely available to view. Please be informed that the free services offered by listed journals may not be available globally. If you find difficulty in viewing the journals listed in our site, please let us know.
125I-Epidermal Growth Factor (EGF) Internalization Assays and Quantification of Surface EGF Receptor (EGFR) and EGFR Degradation
Mol Biol Cell. 2009 July 15; 20(14): 3209-3223
Link to the Protocol

20 S Proteasome Activity Assays
Molecular & Cellular Proteomics 6:2021-2031, 2007
Link to the Protocol

Aconitase and succinate dehydrogenase (SDH) assays
PLoS One. 2009 Oct 29;4(10):e7662
Link to the Protocol

Acridine orange relocation assay
PLoS One. 2009 Nov 18;4(11):e7913
Link to the Protocol

Activated Nuclear Factor (NF)-B Analysis.
Cancer Research 64, 672-677, January 15, 2004
Link to the Protocol

Adenylyl transferase assay
RNA. 2007 August; 13(8): 1235-1244
Link to the Protocol

Adoptive Transfer of T Cells into NOD/scid Mice
PLoS One. 2009 Nov 17;4(11):e7863
Link to the Protocol

Alkaline single-cell microgel electrophoresis assay
Clinical Cancer Research Vol. 11, 5140-5145, July 15, 2005
Link to the Protocol

Alteration of CDR Residues in Selected Antibody Sequences
US Patent#7,619,070
Link to the Protocol

Analysis of drug-inhibition of in vitro liver stage development
PLoS One. 2009 Nov 18;4(11):e7881.
Link to the Protocol

Analysis of the CSF Pathway
PLoS One. 2009 Nov 17;4(11):e7868.
Link to the Protocol

Analysis of ErbB ligand shedding and its effect
Clinical Cancer Research 13, 1892-1902, March 15, 2007
Link to the Protocol

Analysis of Human Cellular Immune Responses to the Antigen
US Patent#7,470,782
Link to the Protocol

Analysis of Possible Anti-Atherogenic Effect of Antibodies are Performed Both in Experimental Animals and in Cell Culture Studies
US Patent#7,468,183
Link to the Protocol

Angiogenesis Assay Using a Matrigel Implant in Mouse
US Patent#7,476,384
Link to the Protocol

Angiogenic Effect on Chick Chorioallantoic Membrane
US Patent#7,476,384
Link to the Protocol

Anti-inflammatory Activity
US Patent#7,371,875
Link to the Protocol

Anti-sperm antibody ELISA :
US Patent#7,204,978
Link to the Protocol

Antiphenylquinone Writhing (PQW) Test
US Patent#6,825,217
Link to the Protocol

Apoptosis-inducing Activity of Humanized Antibody
US Patent#7,476,383
Link to the Protocol

Assay for Contact Sensitivity (CS).
PNAS August 31, 1999 vol. 96 no. 18 10403-10408
Link to the Protocol

Assay for HMG-CoA Reductase Activity
PNAS July 6, 1999 vol. 96 no. 14 7797-7802
Link to the Protocol

Assays of chaperone activity under non-thermal and thermal stresses
Mol Vis. 2009; 15: 1429-1444.
Link to the Protocol

Assessment of M3 mACh Receptor Desensitization by [35S]GTPS Binding.
Molecular Pharmacology Vol. 60, Issue 2, 321-330, August 2001
Link to the Protocol

Assessment of Thyroid Function by Serum Total T4 Levels
PLoS One. 2009 Nov 17;4(11):e7857
Link to the Protocol

ATPase and Helicase Assays
J Biol Chem. 2005 Aug 19;280(33):29604-11
Link to the Protocol

Automated cell-based assay to assess DNA replication.
Cancer Research 65, 7733-7742, September 1, 2005
Link to the Protocol

Biotin surface labeling
Cancer Research 67, 11166-11175, December 1, 2007.
Link to the Protocol

Biotinylation of rat striatal synaptosomes
J Neurosci. 2009 Mar 11;29(10):3328-36
Link to the Protocol

Biotinylation of human DAT-N2A cells
J Neurosci. 2009 Mar 11;29(10):3328-36
Link to the Protocol

Biparameter Flow Cytometry
Cancer Research 64, 6635-6644, September 15, 2004
Link to the Protocol

Blood Flow Simulation
Journal of Biomedicine and BiotechnologyVolume 2009 (2009
Link to the Protocol

C-S lyase activity
Appl Environ Microbiol. 2009 Apr;75(8):2326-32.
Link to the Protocol

Cap-affinity chromatography and western blotting
PLoS One. 2009; 4(9): e7030
Link to the Protocol

Capsaicin Receptor Binding Assay
US Patent#7,432,275
Link to the Protocol

Carboxylesterase activity
Acta Biochim Biophys Sin (Shanghai). 2009 Sep;41(9):731-6.
Link to the Protocol

Carboxypeptidase Y analysis
PLoS ONE. 2008; 3(2): e1553
Link to the Protocol

Cdc42 Activation Assay
Mol Biol Cell. 2009 May;20(9):2413-27
Link to the Protocol

Cell-Based Circadian Assay
PNAS December 30, 2008 vol. 105 no. 52 20746-20751
Link to the Protocol

Cell-based, Functional Assays on Ghrelin Receptor
US Patent#7,476,653
Link to the Protocol

Ceramide Synthase Assays
Molecular Cancer Therapeutics Vol. 1, 719-726, July 2002
Link to the Protocol

Chemical Genomic Screening and Quantitation (Yeast)
Proc Natl Acad Sci U S A. 2005 May 17;102(20):7215-20
Link to the Protocol

Chemotaxis and Ca.sup.2+ Mobilization Assays on Primary Cells
US Patent#7,470,772
Link to the Protocol

Competition Challenge of A. pleuropneumoniae Mutants with Wild Type APP225
US Patent#7,476,391
Link to the Protocol

Confocal Imaging of FITC-labeled Adult Ventricular Myocytes
Molecular & Cellular Proteomics 6:1473-1484, 2007
Link to the Protocol

Conjugation of Alexa 750 PE to phospholipids
Int J Nanomedicine. 2009; 4: 123-131
Link to the Protocol

Covalently Conjugate Antibody to Beads
University of Dundee (UK)
Link to the Protocol

CPC assay for determining amylovoran concentration
BMC Genomics. 2009; 10: 245
Link to the Protocol

Cutinase Activity and Western Blotting
Proc Natl Acad Sci U S A. 2006 February 14; 103(7): 2021-2025
Link to the Protocol

Cys-PEGylation
US Patent#7,619,067
Link to the Protocol

Cytochrome c Oxidase (COX) Activity
PLoS One. 2009 Nov 16;4(11):e7854
Link to the Protocol

Cytosolic ctsB activity assay
PLoS One. 2009 Nov 18;4(11):e7913
Link to the Protocol

Daudi Antiproliferation Assay
US Patent#7,619,067
Link to the Protocol

Decatenation Assay
Clinical Cancer Research Vol. 5, 2899-2907, October 1999
Link to the Protocol

Deimmunization of Selected Antibody Sequences
US Patent#7,619,070
Link to the Protocol

Deoxyhypusine Synthase Assay
JBC Papers in Press. Published on October 30, 2009 as Manuscript M109.048850
Link to the Protocol

Design of Phenotypic Assays and In Vivo Studies for the Use of STAT 6 Inhibitors
US Patent Application#20090292009
Link to the Protocol

Detection of C-S lyase activity by in situ staining
Appl Environ Microbiol. 2009 Apr;75(8):2326-32.
Link to the Protocol

Detection of Murein-Degrading Activity by the Zymogram Technique
Open Microbiol J. 2008;2:107-14.
Link to the Protocol

Detection of ALT-associated promyelocytic leukemia (PML) bodies (APBs)
Clinical Cancer Research Vol. 11, 217-225, January 2005
Link to the Protocol

Determination of ROS production by H2DCFDA fluorescent probe
Mol Pain. 2009 Jun 17;5:31.
Link to the Protocol

Determination of Cytochrome c Release from Intact Cells
Cancer Research 65, 1984-1993, March 1, 2005
Link to the Protocol

Determination of Cytochrome c Release from Isolated Mitochondria
Cancer Research 65, 1984-1993, March 1, 2005
Link to the Protocol

Determination of DNA Interstrand Cross-linking
Clinical Cancer Research Vol. 5, 507-512, March 1999
Link to the Protocol

DHNA-CoA thioesterase assays
Proc Natl Acad Sci U S A. 2009 April 7; 106(14): 5599-5603
Link to the Protocol

DN Thymocyte Enrichment
Molecular & Cellular Proteomics 6:1700-1710, 2007
Link to the Protocol

DNA Damage and Repair
Journal of Biomedicine and BiotechnologyVolume 2009 (2009
Link to the Protocol

dNiR activity assay
Appl Environ Microbiol. 2009 May;75(9):2652-8.
Link to the Protocol

EMS Mutagenesis and Screening Procedures
PLoS One. 2009; 4(6): e6068
Link to the Protocol

Endo-H Resistance Assay
Mol Biol Cell. 2009 May;20(9):2413-27
Link to the Protocol

Enoyl reductase activity assay
PLoS One. 2009 Nov 16;4(11):e7847
Link to the Protocol

Enzymatic Assays of 1-Acenaphthenol Oxidation
Cancer Research 59, 607-614, February 1, 1999
Link to the Protocol

Epitope Tagging
Nucleic Acids Research 2008 36(19):e127
Link to the Protocol

Evaluation of xylanase and esterase activities on agar plates
J Bacteriol. 2009 May; 191(10): 3328-3338
Link to the Protocol

Evaluation of CTL activity by 51Cr-release assays.
Clinical Cancer Research Vol. 11, 4545-4552, June 15, 2005
Link to the Protocol

FcRIIIa-binding assay
Clinical Cancer Research Vol. 12, 2879-2887, May 1, 2006
Link to the Protocol

Filter Retardation Assay
Molecular & Cellular Proteomics 6:781-797, 2007
Link to the Protocol

FITC-induced contact hypersensitivity
International Immunology 2009 21(1):81-93
Link to the Protocol

Fluorescence Polarization (FP) Competition Assays
Molecular Endocrinology 22 (4): 838-857
Link to the Protocol

Fluorescence anisotropy
J Bacteriol. 2009 Feb;191(4):1162-8.
Link to the Protocol

Fluorescence Titration with d-Biotin to Determine Biotin-Binding Ability.
PNAS November 12, 2002 vol. 99 no. 23 14646-14651
Link to the Protocol

Functional Assay for Human GLP-2 Receptor
US Patent#7,473,537
Link to the Protocol

G6PD assay
Genome Biol. 2008; 9(2): R35.
Link to the Protocol

GAP activity assay
EMBO Journal (2007) 26, 3760-3769
Link to the Protocol

GAPDH refolding assay
J Bacteriol. 2009 Feb;191(4):1162-8.
Link to the Protocol

Gelatin Substrate Gel Zymography
Biol. Pharm. Bull. 31(9) 16961703 (2008)
Link to the Protocol

Gelatin zymography and activity of CD13/APN
Clinical Cancer Research Vol. 11, 4610-4619, June 15, 2005
Link to the Protocol

Generation of dendritic cells and peptide pulsing
Clinical Cancer Research Vol. 11, 4545-4552, June 15, 2005
Link to the Protocol

Glutaraldehyde cross-linking
J Bacteriol. 2009 Feb;191(4):1162-8.
Link to the Protocol

GlycoPEGylation of Proteins Expressed in Mammalian or Insect Systems : EPO, Interferon .alpha. and Interferon .beta.
US Patent#7,473,680
Link to the Protocol

GST pull-down assays
Molecular and Cellular Biology, September 2001, p. 6181-6188, Vol. 21, No. 18
Link to the Protocol

GTP?S Binding Studies
Journal of Biomedicine and BiotechnologyVolume 2009 (2009
Link to the Protocol

GTPase Activation Assays
Mol Biol Cell. 2009 July 15; 20(14): 3209-3223
Link to the Protocol

HAT and O-GlcNAcase Assays
J. Biol. Chem., Vol. 279, Issue 51, 53665-53673, December 17, 2004
Link to the Protocol

HBV Screening Assay
US Patent#7,465,742
Link to the Protocol

HeLa-EMCV Antiviral Assay
US Patent#7,619,067
Link to the Protocol

HIF Activation by Hypoxia, Carbon Monoxide or Chemical HIF Inducers in Mice
PLoS One. 2009 Nov 18;4(11):e7875.
Link to the Protocol

High-Throughput Screening Assay for T-Cell Activity
US Patent#7,393,934
Link to the Protocol

High-Throughput Screening Assay Identifying Changes in Small Molecule Concentration and Membrane Permeability
US Patent#7,393,934
Link to the Protocol

HIV-1 Cell Protection Assay
US Patent#7,468,375
Link to the Protocol

HPLC-Based Prolyl 4-Hydroxylase Enzyme Activity Assay
PLoS ONE 4(11): e7635
Link to the Protocol

Human leukocyte chemotaxis assay
US Patent#7,204,978
Link to the Protocol

Identification of Putative MHC Class II-Restricted Epitopes from HLA-DR4-Transgenic Mice
US Patent#7,619,057
Link to the Protocol

Identification of A. pleuropneumoniae Virulence Genes
US Patent#7,476,391
Link to the Protocol

IFNAR Binding Assay
US Patent#7,619,067
Link to the Protocol

Immunoblotting for changes in proteasome target proteins in tumor
Clinical Cancer Research Vol. 11, 3410-3416, May 1, 2005
Link to the Protocol

In Vitro Assays to Determine the Influence of Isolation and Labeling on Monocyte Migratory Function and Activation
PLoS One. 2009 Nov 17;4(11):e7865
Link to the Protocol

In vitro development of liver stages in hepatocyte cultures
PLoS One. 2009 Nov 18;4(11):e7881.
Link to the Protocol

In Vitro Inhibition Assays Using Co-Cultures
PLoS One. 2009 Nov 17;4(11):e7863
Link to the Protocol

In Vitro Propionylation and Butyrylation Assay
Molecular & Cellular Proteomics 6:812-819, 2007.
Link to the Protocol

In Vitro Phosphatase Assay
Molecular & Cellular Proteomics 6:451-459, 2007
Link to the Protocol

In vitro translation and EMSA assays
Nucleic Acids Res. 2009 Sep 25
Link to the Protocol

In Vitro Vid Vesicle-Vacuole Assay
J Biol Chem. 2003 Jul 11;278(28):25688-99
Link to the Protocol

In Vitro Wound-Scratching Assay
PLoS One. 2009; 4(8): e6718.
Link to the Protocol

In-gel CAS activity assay, supershift in gel CAS activity assay, CAS activity assay, OASS activity assay, and SAT activity assay
J Exp Bot. 2009;60(3):993-1008.
Link to the Protocol

Intracerebral Pressure (ICP) Monitoring
PLoS One. 2009 Nov 17;4(11):e7868.
Link to the Protocol

Isolation of 3xFLAG-IMP1 Granules
Molecular & Cellular Proteomics 6:798-811, 2007
Link to the Protocol

Isolation and Staining of Tetramer+ T Cells and Cytokine Assays
PLoS One. 2009 Nov 17;4(11):e7863
Link to the Protocol

Kynurenine aminotransferase assay
Mol Cell Biol. 2009 Feb;29(3):784-93
Link to the Protocol

Long SAGE (Serial Analysis of Gene Expression) of CD45 Negative Mouse Thyrocytes
PLoS One. 2009 Nov 17;4(11):e7857
Link to the Protocol

Longevity and aging assays (C. elegans )
PLoS One. 2009 Nov 16;4(11):e7847
Link to the Protocol

Making Methyl Esters
Ionsource
Link to the Protocol

Measurement of RalA activation using a pull-down assay
PLoS ONE 4(11): e7770
Link to the Protocol

Measurement of Pharmacokinetic (PK) and Pharmacodynamic (PD) Profiles of a Polypeptide or Conjugate of the Invention
US Patent#7,619,067
Link to the Protocol

Measurement of Serum Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST)
PLoS Pathog. 2009 May; 5(5): e1000440
Link to the Protocol

Measurement of IGF-IR, GAPDH, HPRT, Insulin Receptor (IR), Caspase 3 & Bax mRNA Levels by Real-Time PCR
US Patent#7,468,356
Link to the Protocol

Measurement of Mitochondrial Membrane Potential in Intact Cells
Cancer Research 65, 1984-1993, March 1, 2005
Link to the Protocol

Metal ion-binding assay
J Virol. 2008 June; 82(11): 5279-5294.
Link to the Protocol

Method for Full Protein Sequence Mapping
Cell Migration Consortium
Link to the Protocol

Microtubule cosedimentation assay
PLoS One. 2009 Oct 1;4(10):e7239.
Link to the Protocol

Mitochondrial Oxygen Consumption
J Pharmacol Exp Ther311:668-676, 2004
Link to the Protocol

Mitochondrial Respiration
PLoS One. 2009 Nov 16;4(11):e7854
Link to the Protocol

Mouse Hypercalcemia Assay
US Patent Application#20090018058
Link to the Protocol

Mouse phrenic nerve hemidiaphragm assay (MPNHDA)
Antimicrob Agents Chemother. 2009 August; 53(8): 3478-3486
Link to the Protocol

Myelin vesicles (MV) internalization assays
Journal of Cell Science 122, 1155-1162 (2009)
Link to the Protocol

Myocardial Infarction and BrdU labeling
Circ Res. 2009 Jan 2;104(1):e1-7.
Link to the Protocol

N-Terminal PEGylation
US Patent#7,619,067
Link to the Protocol

Orexin-1 Receptor Antagonist Activity
US Patent#7,470,710
Link to the Protocol

Palmitoyl-CoA Synthetase Assays
Molecular Cancer Therapeutics Vol. 1, 719-726, July 2002
Link to the Protocol

PC-I Release Assay
Mol Biol Cell. 2009 May;20(9):2413-27
Link to the Protocol

Pegylation of Interferon-Alpha Polypeptides
US Patent#7,619,067
Link to the Protocol

Pericyte c-Fos Induction
US Patent#7,476,723
Link to the Protocol

Peripheral Blood Micronucleus Test
Journal of Biomedicine and BiotechnologyVolume 2009 (2009
Link to the Protocol

Phosphate uptake analysis
EMBO Journal (2007) 26, 4029-4037
Link to the Protocol

Phospholipase activity assays
Cancer Research 65, 8286-8297, September 15, 2005
Link to the Protocol

Platelet aggragation assay by WBA Carna
Mol Cell Proteomics. 2008 Aug 11
Link to the Protocol

Poly(ADP-ribose) polymerase assay procedure
Clinical Cancer Research Vol. 11, 3402-3409, May 1, 2005
Link to the Protocol

Porcine Screening for Attenuated A. pleuropneumoniae Mutants
US Patent#7,476,391
Link to the Protocol

Preparation of Cytoplasmic and Microsomal Fractions and Detection of PKC Activation
PLoS One. 2009 Nov 18;4(11):e7889
Link to the Protocol

Preparation of Bovine Pituitary Membranes and GnRH Receptor Binding Assay
Cancer Research 64, 2090-2095, March 15, 2004
Link to the Protocol

Preparation of Microsomes and Assays
Cancer Research 63, 2752-2761, June 1, 2003
Link to the Protocol

Preparation of Mitotic Extracts for Mitotic Aster Formation
Proc Natl Acad Sci U S A. 2008 October 7; 105(40): 15441-15445
Link to the Protocol

Preparation of Nuclear Extracts and Electrophoretic Mobility Shift Assay (EMSA)
JBC Volume 270, Number 41, Issue of October 13, 1995 pp. 24435-24441
Link to the Protocol

Preparation of Synchronized HeLa Cell Extracts.
Proc Natl Acad Sci U S A. 2008 April 22; 105(16): 6069-6074
Link to the Protocol

Preparation of a Galectin 6 Column
Molecular & Cellular Proteomics 6:2100-2109, 2007.
Link to the Protocol

Preparation of Vid Vesicles, Vacuoles, and Cytosol
J Biol Chem. 2003 Jul 11;278(28):25688-99
Link to the Protocol

Protocol for Serum Cav-1 Assay (biomarker for prostate cancer )
US Patent#7,462,491
Link to the Protocol

Rac Biosensor
PLoS One. 2009 Nov 18;4(11):e7886
Link to the Protocol

RCM-RNase T1 refolding assay
J Bacteriol. 2009 Feb;191(4):1162-8.
Link to the Protocol

Ribohomopolymer binding assay
Plant Physiol. 2009 Nov 6
Link to the Protocol

RXR-VDR heterodimerAssay
US Patent Application#20090018058
Link to the Protocol

scFv2H12 export monitoring
J Bacteriol. 2009 Feb;191(4):1162-8.
Link to the Protocol

Screening assay for differentiation inducers
Cancer Research 65, 7847-7855, September 1, 2005
Link to the Protocol

Signal transducers and activators of transcription 3 activity assay
Clinical Cancer Research Vol. 11, 4589-4600, June 15, 2005
Link to the Protocol

Simulator of the Human Intestinal Microbial Ecosystem (SHIME)
Journal of Biomedicine and BiotechnologyVolume 2009 (2009),
Link to the Protocol

Single-cell gel electrophoresis.
Clinical Cancer Research Vol. 11, 7047s-7052s, October 1, 2005
Link to the Protocol

Single-channel current recording
Mol Pain. 2009 Feb 10;5:5.
Link to the Protocol

Skin Vascular Permeability Assay
US Patent#7,473,763
Link to the Protocol

Small-molecule high-throughput screening (Anti-cancer)
Clinical Cancer Research Vol. 12, 5557-5569, September 15, 2006
Link to the Protocol

Solid-phase binding assay with purified integrins.
Cancer Research 66, 5807-5815, June 1, 2006
Link to the Protocol

Synaptic current recording
Mol Pain. 2009 Feb 10;5:5.
Link to the Protocol

T.sub.H1 Differentiation Assay
US Patent#7,619,067
Link to the Protocol

Thermal hyperalgesia assay
PLoS One. 2009; 4(10): e7596
Link to the Protocol

Transient Replication Assays with Luciferase Replicons
J Biol Chem. 2005 Aug 19;280(33):29604-11
Link to the Protocol

Tumor Regression Experiments in Nude Mice
PLoS One. 2009 Nov 18;4(11):e7889
Link to the Protocol

TUNEL Staining to Detect Apoptosis in Mouse Thyroid Glands
PLoS One. 2009 Nov 17;4(11):e7857
Link to the Protocol

Two step biotinylated DNA-binding assay
University of Texas Medical Branch Galveston (USA)
Link to the Protocol

Whole Cell Competition Binding Assay
Journal of Biomedicine and BiotechnologyVolume 2009 (2009
Link to the Protocol

Whole-blood tryptophan and 5-HT measurement
BMC Physiol. 2009 Mar 25;9:4
Link to the Protocol

Whole-cell current recording
Mol Pain. 2009 Feb 10;5:5.
Link to the Protocol

X chromosome inactivation studies
J Clin Invest. 2008 March 3; 118(3): 904-912.
Link to the Protocol

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