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We have a unique collection of protocols from journals and patents. We have listed thousands of protocols and in most cases, the titles of protocols listed in our site are similar to the "Materials & Methods" section of the journals. Sometimes, it is not easy to categorize these protocols. We are in the process of categorizing these protocols. Meantime, we strongly recommend you to search for the protocols using the search function.
Bioprotocols > Immunocytochemistry/Immunochemistry

Immunocytochemistry/Immunochemistry Protocols

The protocols listed in this section are extracted from scientific journals or patents or laboratory websites. We have cited only the journals that are freely available to view. Please be informed that the free services offered by listed journals may not be available globally. If you find difficulty in viewing the journals listed in our site, please let us know.
2-Step Immunoperoxidase Procedure : Formalin-fixed, Paraffin-embedded tissues and cell blocks
Bethyl Laboratories
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2-Step Immunoperoxidase Procedure : Epitope Retrieval Method for Formalin-fixed, Paraffin-embedded tissues and cell blocks
Bethyl Laboratories
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2-Step Immunoperoxidase Protocol : Formaldehyde-Fixed Cells and Cytospin Preparations
Bethyl Laboratories
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2-Step Immunoperoxidase Protocol : Cells Grown in Culture and Cytospin Preparations
Bethyl Laboratories
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2-Step Immunofluorescence Protocol : Formalin-fixed, Paraffin-embedded tissues and cell blocks
Bethyl Laboratories
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2-Step Immunofluorescence Protocol : Epitope Retrieval Method for Formalin-fixed, Paraffin-embedded tissues and cell blocks
Bethyl Laboratories
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2-Step Immunofluorescence Protocol : Fresh Frozen Tissue Sections
Bethyl Laboratories
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Analysis of Pancreatic Related Proteins by Immunohistochemistry
US Patent Application#20090280096
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Antigen Retrieval Protocols
Neuromics
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Brain Tissue Preparation, Immunohistochemical Analyses, and Quantitation (Mice)
PLoS One. 2009 Nov 17;4(11):e7868.
Link to the Protocol

Cardiomyocyte immunofluorescence protocol
Abcam
Link to the Protocol

Cell Movement by Histology
Investigative Ophthalmology & Visual Science, January 2005, Vol. 46, No. 1
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Cell Movement by In Vivo Microscopy
Investigative Ophthalmology & Visual Science, January 2005, Vol. 46, No. 1
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Cell Surface Immunofluorescence Staining Protocol
BMA Biomedicals
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Characterization of definitive endoderm with immunocytochemistry and quantitative analysis
WO/2009/013254
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Combined Fluorescence in Situ Hybridization (FISH) and immunofluorescence analysis (endothelial differentiation of human breast tumor cells)
J Cell Mol Med. 2008 Apr 9.
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Confocal immunocytochemistry procedures
Mol Vis. 2009; 15: 2384-2403
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Cryo-EM immunolocalization
J Exp Bot. 2009;60(3):993-1008.
Link to the Protocol

Double immunocytochemical analysis and confocal microscopy
J Neurosci. 2008 Apr 16;28(16):4250-60
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Double immunofluorescence : sequential protocol
Abcam
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Double immunofluorescence : simultaneous protocol
Abcam
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Double label immunohistochemistry
Finger Lab (USA)
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Evaluation of Immune Cell Densities
PLoS One. 2009 Nov 16;4(11):e7847
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Fixation and permeabilization for IHC and ICC
Abcam
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Fluorescence in situ hybridization
Hum. Reprod., Oct 2008; 23: 2185 - 2193
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Fluorescent Immunohistochemistry Assay
Stem Cells Vol. 26 No. 9 September 2008, pp. 2245 -2256
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General Guidelines For Immunostaining
Meridian Lifescience
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GMA embedding for immmunohistochemistry
Abcam
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Golgi staining
Biochem J. 2009 August 15; 422(Pt 1): 23-35
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IHC frozen sections staining protocol
Abcam
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IHC paraffin staining protocol
Abcam
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IHC Protocol for Free Floating Brain Sections
Abcam
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Immunoblotting of differentiating mES cells
J Neurosci. 2008 Apr 16;28(16):4250-60
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Immunocytochemistry (Human Neural Stem Cells)
J Vis Exp. 2007; (7): 267.
Link to the Protocol

Immunocytochemistry (Cardiomyocyte differentiation)
BBMB Rep. 2008 Oct 31;41(10):699-704
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Immunocytochemistry of ESCs
ATCC
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Immunocytochemistry (Pancreatic differentiation of BM cells)
Endocrine Journal Vol. 55 (2008) , No. 3 535-543
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Immunocytochemistry and Quantification of In Vitro Differentiation (Rat and Human Olfactory Mucosa)
Stem Cells Vol. 26 No. 8 August 2008, pp. 2183 -2192
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Immunocytochemical Identification of Nestin Positive Human and Rat Pancreatic Stem Cells
US Patent#7,438,902
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Immunocytochemistry of Retinal Stem Cells (RSCs)
Investigative Ophthalmology and Visual Science. 2008;49:429-437
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Immunofluorescence Microscopy of Cells and Embryos
Stem Cells, Aug 2008; 26: 2019 - 2031
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Immunofluorescence (endothelial differentiation of human breast tumor cells)
J Cell Mol Med. 2008 Apr 9.
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Immunofluorescence (Differentiation of ESCs into Insulin-Producing Cells)
Stem Cells Vol. 26 No. 8 August 2008, pp. 1939 -1950
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Immunofluorescence Microscopy Protocol
BMA Biomedicals
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Immunohistochemical analysis of markers of neurogenesis
J Neurosci. 2008 Apr 16;28(16):4250-60
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Immunohistochemistry (adult pituitary stem cells)
Proc Natl Acad Sci U S A. 2008 April 29; 105(17): 63326337
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Immunohistochemistry with fresh frozen tissue sections
BMA Biomedicals
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Immunohistochemistry with paraffin sections
BMA Biomedicals
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Immunohistochemistry with fresh frozen tissue sections and biotinylated antibodies
BMA Biomedicals
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Immunohistochemistry with fresh frozen tissue sections and chicken antibodies
BMA Biomedicals
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Immunohistochemistry Protocol for Frozen Sections
BMA Biomedicals
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Immunohistochemistry Protocol for Paraffin-Embedded Sections
BMA Biomedicals
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Immunohistochemistry Accessory Kit Protocol
Bethyl Laboratories
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Immunohistochemistry Protocols
Neuromics
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Immunohistochemistry Protocol for Paraffin-embedded Tissues
Epitomics
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Immunohistochemistry (Limbal epithelial cells)
Indian J Med Res 128, August 2008, pp 149-156
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Immunohistochemical analysis (Mouse tissues)
PLoS ONE. 2009; 4(4): e4992
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Immunohistochemical Characterization of Cell Phenotypes (Human umbilical cord tissue)
US Patent Application#20090092653
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Immunohistochemical Staining of PSCA in Normal Prostate
US Patent#7,485,296
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Immunohistochemical Identification of Collagen Type I, Collagen Type II, or Ki67 in Tissue
US Patent Application#20090074835
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Immunohistochemical Characterization of Postpartum Tissue Phenotypes
US Patent#7,524,489
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Immunohistochemical Staining
PLoS One. 2009 Nov 16;4(11):e7847
Link to the Protocol

Immunophenotyping analysis (Peripheral blood stem cells)
Blood, Vol 85, No 7 (April I), 1995: pp 1964-1970
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In situ tetramer and immunohistochemical staining
PLoS ONE. 2009; 4(1): e4131.
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In Vitro Immunological Evaluation of Postpartum-Derived Cells
US Patent#7,524,489
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Indirect Immunofluorescence
J Biol Chem. 2008 Nov 17
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Indirect immunofluorescence
PLoS Pathog. 2009 August; 5(8): e1000546
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Intravital fluorescence microscopy
J Neurosci. 2008 Apr 23;28(17):4406-13
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Laser-scanning confocal microscopy analysis
J Virol. 2008 Dec;82(24):12487-97
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Lipid dyes
Biochem J. 2009 August 15; 422(Pt 1): 23-35
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MAbs against Human MSCs
US Patent#7,476,540
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Measurement of Cell Area Sizes in Sections with Isolated CD3 Positive T Cells
PLoS One. 2009 Nov 16;4(11):e7847
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OCT4 Western Blot and Immunohistochemistry
Experimental Biology and Medicine 232:1368-1380 (2007)
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Plasma membrane staining
Biochem J. 2009 August 15; 422(Pt 1): 23-35
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Preparing cells and sections for BrdU immunostaining
Abcam
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Primary Cultures for IHC  Viability Assays
Abcam
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Sample Immunohistochemistry Protocol (Frozen Sections)
Neuromics
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Sample Immunohistochemistry Protocol (Paraffin Sections)
Neuromics
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Sample Immunohistochemistry Protocol for Chicken Neuron/Glial Markers
Neuromics
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Sequential Immunoperoxidase Labeling and Erasing Method
J Histochem Cytochem. 2009 October; 57(10): 899-905.
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Standard Immunofluoresence Protocol
Neuromics
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Whole Mount Non-radioactive In Situ Hybridization On Mouse Embryos
Finger Lab (USA)
Link to the Protocol

Whole mount electro-immunofluorescence staining
Mol Vis. 2009; 15: 505-517.
Link to the Protocol

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