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Bioprotocols > Miscellaneous Assays and Methods

Miscellaneous Assays and Methods Protocols

The protocols listed in this section are extracted from scientific journals or patents or laboratory websites. We have cited only the journals that are freely available to view. Please be informed that the free services offered by listed journals may not be available globally. If you find difficulty in viewing the journals listed in our site, please let us know.
Affinity-binding assay for RhoA-GTP
Am J Physiol Cell Physiol. 2008 November; 295(5): C1161-C1168
Link to the Protocol

Analysis of drug-inhibition of in vitro liver stage development
PLoS One. 2009 Nov 18;4(11):e7881
Link to the Protocol

Analysis of the inhibition of in vivo liver stage development by primaquine and tafenoquine
PLoS One. 2009 Nov 18;4(11):e7881
Link to the Protocol

Analysis of membrane ruffling
J Leukoc Biol. 2009 Nov 17. [Epub ahead of print]
Link to the Protocol

Arrested Cell Assay
US Patent#7,605,175
Link to the Protocol

Assay of purine nucleoside phosphorylase activity
Bioorg Chem. 2008 April; 36(2): 65-69.
Link to the Protocol

Assays for Antagonist Activity of Anti-CD40 Therapeutic Agents
US Patent Application#20080274118
Link to the Protocol

B-Cell Proliferation Assay.
US Patent Application#20080274118
Link to the Protocol

Banchereau-Like B-Cell Proliferation Assay
US Patent Application#20080274118
Link to the Protocol

Borate Wash Method for Purifying Recombinant Con A Variants from E. coli.
US Patent#7,619,072
Link to the Protocol

Brain Histology and Immunohistochemistry
PLoS One. 2009 Nov 18;4(11):e7900
Link to the Protocol

Caco-2 cell Co-transfection Assay
US Patent Application#20090018058
Link to the Protocol

Cell adhesion assay
Clin Cancer Res. 2008 Aug 15;14(16):5069-80.
Link to the Protocol

Cell Conjugation Assay
PNAS February 19, 2008 vol. 105 no. 7 2568-2573
Link to the Protocol

Cell Cycle Analysis Using Bromodeoxyuridine Labeling
Mol Cancer Ther. 2004;3:849-860
Link to the Protocol

Cell migration assay
Clin Cancer Res. 2008 Aug 15;14(16):5069-80.
Link to the Protocol

Cell Surface Biotinylation
Mol Biol Cell. 2008 September; 19(9): 3859-3870.
Link to the Protocol

Characterization of LctD-his6 Enzymatic Activity (L-Lactate Dehydrogenase)
PLoS One. 2009 Nov 17;4(11):e7864.
Link to the Protocol

Characterization of ATX Kinase Activity
Proc Natl Acad Sci U S A. 2008 April 15; 105(15): 5809-5814
Link to the Protocol

Chromaffin Cell Release Experiments
Molecular & Cellular Proteomics 6:1158-1169, 2007
Link to the Protocol

Chromatographic Assay for Chronic Alcoholism
US Patent#5,747,346
Link to the Protocol

Chromogenic Peptide Substrate Protease Assay (CPSPA).
US Patent#6,753,155
Link to the Protocol

Coat Recruitment Assay
Mol Biol Cell. 2008 May; 19(5): 1942-1951.
Link to the Protocol

Confocal microscopy for NF-kB localization
Br J Pharmacol. 2008 Jun;154(4):852-63
Link to the Protocol

Confocal microscopy for surface molecules
Br J Pharmacol. 2008 Jun;154(4):852-63
Link to the Protocol

Cytosolic ctsB activity assay
PLoS One. 2009 Nov 18;4(11):e7913
Link to the Protocol

Detection of Serum Immunoglobulin Subclasses
Cancer Research 60, 2449-2457, May 1, 2000
Link to the Protocol

Determination of mACh Receptor Number and Receptor Internalization
Molecular Pharmacology Vol. 60, Issue 2, 321-330, August 2001
Link to the Protocol

Determination of Mitochondrial Swelling
Cancer Research 65, 1984-1993, March 1, 2005
Link to the Protocol

Determination of ATP, ADP, and AMP Concentrations
BMC Dev Biol. 2009; 9: 21.
Link to the Protocol

Determine the MHC Binding Ability of Selected Peptides
US Patent#6,861,234
Link to the Protocol

DNA Adduction and Isolation
Cancer Research 61, 103-109, January 1, 2001
Link to the Protocol

DNA Cell Cycle Analysis
Mol Cancer Ther. 2006;5:296-308
Link to the Protocol

DNA damage analysis
PLoS Genet. 2009 Dec;5(12):e1000749. Epub 2009 Dec 4
Link to the Protocol

DNA integrity analysis
Clinical Cancer Research Vol. 12, 4569-4574, August 1, 2006
Link to the Protocol

DNA repair assays
PLoS Genet. 2009 Dec;5(12):e1000749. Epub 2009 Dec 4
Link to the Protocol

Dorsal Root Ganglion Cell Assay
US Patent#7,432,275
Link to the Protocol

Drug Sensitivity (Colony Formation) Assay
Mol Cancer Ther. 2006;5:29-38
Link to the Protocol

Dual Imaging of FRET-Based Indicators and Calcium
PLoS One. 2009; 4(11): e7877.
Link to the Protocol

Electrophoretic Mobility Shift Assay (nuclear factor B (NF-B) binding)
Mol Cancer Ther. 2006;5:230-237
Link to the Protocol

Electrophoretic mobility shift assay for measuring NF-B activity
Clinical Cancer Research Vol. 11, 6075-6086, August 15, 2005
Link to the Protocol

Electrophoretic mobility shift assay for measuring nuclear factor-B activity
Cancer Research 65, 3877-3882, May 1, 2005
Link to the Protocol

Endothelial Cell Tube Formation Assay
US Patent#7,585,967
Link to the Protocol

Ex vivo B-cell depletion assay.
Clinical Cancer Research Vol. 12, 2879-2887, May 1, 2006
Link to the Protocol

Exoglycosidase treatment of purified IgG
US Patent Application#20090253180
Link to the Protocol

Experimental Autoimmune Encephalomyelitis (EAE)
US Patent Application#20080268479
Link to the Protocol

Fluorescence Assay for Intracellular Presence of NF-B.
Clinical Cancer Research Vol. 9, 1136-1144, March 2003
Link to the Protocol

Fluorescence-Activated Cell Sorting Analysis for Poly(ADP-Ribose) Polymerase-Positive Cells
Mol Cancer Ther. 2006;5:29-38
Link to the Protocol

G418-resistant colony formation assay.
Cancer Research 66, 5847-5857, June 1, 2006
Link to the Protocol

Human T Cell Helper Assay for Antibody Production by B Cells.
US Patent Application#20080274118
Link to the Protocol

Immunoprecipitation from Hippocampal Synaptic Fractions
Molecular & Cellular Proteomics 6:29-42, 2007.
Link to the Protocol

Immunoprecipitation of cohesin-associated plasmids
Nucleic Acids Res. 2009 Nov 17. [Epub ahead of print]
Link to the Protocol

Immunoprecipitation
PLoS One. 2009; 4(11): e7882.
Link to the Protocol

In vitro B-cell depletion assay
Clinical Cancer Research Vol. 12, 2879-2887, May 1, 2006
Link to the Protocol

In Vitro Cell-Based Assay for Inhibitors of Lethal Toxin-Mediated Cytotoxicity
US Patent#7,276,530
Link to the Protocol

In vitro development of liver stages in hepatocyte cultures
PLoS One. 2009 Nov 18;4(11):e7881
Link to the Protocol

In vitro Growth Inhibition Assay
Mol Cancer Ther. 2006;5:2634-2643
Link to the Protocol

In Vitro Guanine Nucleotide Exchange Analysis
J Biol Chem. 2008 October 17; 283(42): 28392-28400
Link to the Protocol

In vitro metalloprotease and pharmacology assays
Clinical Cancer Research 13, 1892-1902, March 15, 2007
Link to the Protocol

In vitro parasite growth inhibition (Plasmodium falciparum)
PLoS ONE. 2008; 3(12): e3960
Link to the Protocol

In Vitro Transport Assays
Cancer Research 62, 6158-6164, November 1, 2002
Link to the Protocol

In Vitro Wound Closure Assay
Cancer Research 62, 6329-6336, November 1, 2002
Link to the Protocol

In vivo acetylation assays
Molecular and Cellular Biology, September 2001, p. 6181-6188, Vol. 21, No. 18
Link to the Protocol

In Vivo Data Rat Penile Erection Model
US Patent#7,470,691
Link to the Protocol

In Vivo Hollow Fiber Test
Cancer Research 61, 4030-4037, May 15, 2001]
Link to the Protocol

In Vivo Inhibition of Cellular Muscle Breakdown
US Patent#7,468,383
Link to the Protocol

In Vivo Investigation of Anti-Tumor Activity
US Patent#7,468,383
Link to the Protocol

In Vivo Rabbit Eye Model Assay
US Patent#7,585,841
Link to the Protocol

In Vivo Tumor Efficacy Model
US Patent#7,442,830
Link to the Protocol

In vivo tumorigenicity assays
Clinical Cancer Research 13, 5926-5934, October 1, 2007
Link to the Protocol

Induction of antigen-specific T-cell responses
Clinical Cancer Research Vol. 11, 4545-4552, June 15, 2005
Link to the Protocol

Induction of c-fos in Endothelial Cells
US Patent#7,473,763
Link to the Protocol

Inhibition of NF-.kappa.B-mediated Luciferase Activity
US Patent#7,276,530
Link to the Protocol

Integrase Strand-Transfer Scintillation Proximity Assay Oligonucleotides
US Patent#7,468,375
Link to the Protocol

Invasion assay
Clinical Cancer Research Vol. 12, 5910-5918, October 1, 2006
Link to the Protocol

Isolation of Ingested/Attached Yeasts after Macrophage Interaction
Molecular & Cellular Proteomics 6:460-478, 2007
Link to the Protocol

Isolation and Analysis of Nuclear Matrix Proteins and Proteins Cross-linked to DNA in Situ.
Cancer Research 60, 288-292, January 15, 2000
Link to the Protocol

Isolation of CD138+ myeloma cells
Clinical Cancer Research Vol. 11, 4589-4600, June 15, 2005
Link to the Protocol

Isolation of lipid raft microdomains, lipid raft staining, and cholesterol assays
Cancer Research 67, 11166-11175, December 1, 2007
Link to the Protocol

Isolation of lipid raft microdomains, lipid raft staining, and cholesterol assays.
Cancer Research 67, 11166-11175, December 1, 2007.
Link to the Protocol

Isolation of Lytic Granules.
PNAS February 19, 2008 vol. 105 no. 7 2568-2573
Link to the Protocol

Isolation of peripheral blood mononuclear cells (PBMCs)
US Patent Application#20090018058
Link to the Protocol

Isolation of the Full-Length Human GLP-2 Receptor cDNA
US Patent#7,473,537
Link to the Protocol

Keratinocyte Proliferation Assay
US Patent Application#20090018058
Link to the Protocol

Kv1.3-Assay
US Patent#7,317,030
Link to the Protocol

Lectin Fluorophore-Linked Immunosorbent Assay (FLISA)
US Patent Application#20090253180
Link to the Protocol

Lipid and lysoPLD measurements
US Patent#7,198,928
Link to the Protocol

Lipid extraction, electrospray ionization/mass spectrometry, and atmosphere pressure chemical ionization analysis of lipids
Cancer Research 66, 5807-5815, June 1, 2006
Link to the Protocol

Lipid extraction
US Patent#7,198,928
Link to the Protocol

Lymphadema Animal Model
US Patent#7,476,384
Link to the Protocol

Lymphocyte isolation and treatment of cells with phytohemagglutinin
Clinical Cancer Research Vol. 11, 5140-5145, July 15, 2005
Link to the Protocol

Lymphoproliferative Assay
Cancer Research 62, 5770-5777, October 15, 2002
Link to the Protocol

Madin-Darby canine kidney cell scattering assay
Clinical Cancer Research Vol. 12, 1292-1298, February 2006
Link to the Protocol

Matrigel invasion assay
Clinical Cancer Research 13, 2335-2343, April 15, 2007
Link to the Protocol

Measurement of cell cycle distribution
Clinical Cancer Research Vol. 11, 7047s-7052s, October 1, 2005
Link to the Protocol

Measurement of IGF-IR mRNA Levels by Real-Time PCR
US Patent#7,468,356
Link to the Protocol

Measuring Carboxylase (CE) Activity in Saliva and EC.sub.50 for Inhibition by Pesticides
US Patent#6,537,744
Link to the Protocol

Megakaryocyte Culture and Purification
Molecular & Cellular Proteomics 6:548-564, 2007
Link to the Protocol

Metabolic Stability in Human Plasma
US Patent#7,476,653
Link to the Protocol

Metabolic Pulse-Chase Labeling
Mol Biol Cell. 2008 September; 19(9): 3859-3870.
Link to the Protocol

Metal affinity resin pulldown assay
Journal of Virology, January 2009, p. 37-46, Vol. 83, No. 1
Link to the Protocol

Methylene Blue Growth Inhibition Assay
US Patent#7,452,906
Link to the Protocol

Miccrococcal Nuclease Digestion
PLoS One. 2009; 4(12): e8111
Link to the Protocol

Minimal inhibitory concentration (MIC) asay for venoms
Evid Based Complement Alternat Med. 2008 Sep 2
Link to the Protocol

Mitochondrial and cytosolic fractionation
Cancer Research 67, 756-764, January 15, 2007.
Link to the Protocol

Mitochondrial depolarization, calcium content, and propidium iodide uptake
Clinical Cancer Research Vol. 12, 5557-5569, September 15, 2006
Link to the Protocol

Mitochondrial Swelling
J Pharmacol Exp Ther311:668-676, 2004
Link to the Protocol

Modeling using Ingenuity pathway analysis
BMC Syst Biol. 2008; 2: 19.
Link to the Protocol

Monitoring the GTPase Activity of GPCRs using GTP.beta.s in Dithio-coupled Assays
US Patent#7,585,643
Link to the Protocol

Monoclonal antibody labeling
Clinical Cancer Research Vol. 11, 7047s-7052s, October 1, 2005
Link to the Protocol

Motilin Agonist Potency (Tissue Based Assay)
US Patent#7,211,568
Link to the Protocol

Mouse Corneal Micropocket Assay
US Patent#7,585,967
Link to the Protocol

Murine Matrigel Plug Angiogenesis Assay
US Patent#7,585,967
Link to the Protocol

NF-B ELISA assay
EMBO Journal (2008) 27, 1671-1681
Link to the Protocol

NK (LGL) Cell Isolation
US Patent#7,393,534
Link to the Protocol

Normal Human Dermal Fibroblast Proliferation
US Patent#7,476,723
Link to the Protocol

Nuclear translocation assay
Cancer Research 66, 3558-3566, April 1, 2006
Link to the Protocol

OCN (osteocalcin) Promoter Assay
US Patent Application#20090018058
Link to the Protocol

Oligonucleosome Reconstitution
J. Biol. Chem., Vol. 279, Issue 51, 53665-53673, December 17, 2004
Link to the Protocol

Osteoclast differentiation assay
Clinical Cancer Research Vol. 12, 5910-5918, October 1, 2006
Link to the Protocol

Osteoclast-Like Cell Formation Assay
Mol Cancer Ther. 2006;5:2634-2643
Link to the Protocol

p16INK4a CDK4 Binding Assays.
Clinical Cancer Research Vol. 7, 3282-3288, October 2001
Link to the Protocol

Parasite culture and mosquito infection (Plasmodium)
Malar J. 2009; 8: 99.
Link to the Protocol

PEP carboxylase assay
Proc Natl Acad Sci U S A. 2009 Nov 16. [Epub ahead of print]
Link to the Protocol

Phagocytosis Quantification (Yeast)
Molecular & Cellular Proteomics 6:460-478, 2007
Link to the Protocol

Phosphatase assay
Protein Sci. 2008 November; 17(11): 1979-1986
Link to the Protocol

Plasmodium falciparum amino acid starvation assay
Malar J. 2009; 8: 99.
Link to the Protocol

Preparation of parasite pellets (Plasmodium falciparum )
Malar J. 2009; 8: 99.
Link to the Protocol

Procedure for Measuring Acetylcholinesterase (AChE) Activity in Saliva and EC.sub.50 for Inhibition by Pesticides
US Patent#6,537,744
Link to the Protocol

Production of Humanized Antibody
US Patent#7,476,383
Link to the Protocol

Production of Recombinant Murine, Humanized and Human Monoclonal Antibodies in PER.C6.TM. and PER.C6.TM./E2A Cells
US Patent#7,470,523
Link to the Protocol

Production, purification, and analysis of recombinant candidate regulators ((B. burgdorferi)
Nucleic Acids Res. 2009 Nov 12. [Epub ahead of print]
Link to the Protocol

Protein Degradation and Release Assay
Mol. Biol. Cell Vol. 17, Issue 12, 4962-4971, December 2006
Link to the Protocol

Protein Misfolding Cyclic Amplification (PMCA)
PLoS One. 2009; 4(11): e7816.
Link to the Protocol

Protein translocation assay (Bacterial)
J Bacteriol. 2008 May; 190(10): 3505-3525
Link to the Protocol

Proteomcis identification of antitumor drug activity
Molecular Cancer Therapeutics 6, 1898-1908, June 1, 2007
Link to the Protocol

Purification Scheme of Apoptosis-inducing Factors
Molecular & Cellular Proteomics 6:1239-1247, 2007
Link to the Protocol

Purification and glycan analysis of anti-gal IgG
US Patent Application#20090253180
Link to the Protocol

Quantification of cGMP
US Patent#7,119,120
Link to the Protocol

Rac activation assay
J Leukoc Biol. 2009 Nov 17. [Epub ahead of print]
Link to the Protocol

Ras Activity Assay and Immunoblotting
Cancer Research 64, 4577-4584, July 1, 2004
Link to the Protocol

Rat Smooth Muscle Cell Proliferation Assay
US Patent#7,476,688
Link to the Protocol

Reactive oxygen species detection
Clinical Cancer Research Vol. 11, 5248-5256, July 15, 2005
Link to the Protocol

Red Marrow Dosimetry
US Patent#7,465,551
Link to the Protocol

Repression assays
Molecular and Cellular Biology, September 2001, p. 6181-6188, Vol. 21, No. 18
Link to the Protocol

Rescue of Ischemia in Rabbit Lower Limb Model
US Patent#7,476,384
Link to the Protocol

Ret Activity Assay
Proc Natl Acad Sci U S A. 2008 April 15; 105(15): 5809-5814
Link to the Protocol

RH1 Antitumor Activity.
Clinical Cancer Research Vol. 10, 3147-3155, May 1, 2004
Link to the Protocol

RhoA Activation Assay
Cancer Epidemiology Biomarkers & Prevention 17, 88-94, January 1, 2008
Link to the Protocol

S-Phase Checkpoint Assays
Cancer Research 64, 6635-6644, September 15, 2004
Link to the Protocol

Salmonella Infection Assay
BMC Microbiol. 2009; 9: 218
Link to the Protocol

Simple Qualitative Assay for Chronic Alcohloism
US Patent#5,747,346
Link to the Protocol

Small Interfering RNA (siRNA) Treatment and Antibody Uptake Assay
Mol Biol Cell. 2008 May; 19(5): 1942-1951.
Link to the Protocol

Solid Phase Sandwich Microtiter ELISA for Human Serum and Plasma Sample Preparation
US Patent Application # 20080311604
Link to the Protocol

SPT Assays
Molecular Cancer Therapeutics Vol. 1, 719-726, July 2002
Link to the Protocol

Standard 51Cr-release assays (CTL assays).
Clinical Cancer Research 13, 3347-3355, June 1, 2007
Link to the Protocol

STAT ELISA-based EMSA assay
J Cell Sci. 2008 Feb 1;121(Pt 3):272-81
Link to the Protocol

Stimulatory Activity in Mixed Lymphocyte Reaction (MLR)
US Patent#7,473,763
Link to the Protocol

Subcellular localization assays
Nucleic Acids Res. 2008 March; 36(4): 1209-1219
Link to the Protocol

Surrogate Marker for HDAC Activity in Rat Peripheral Blood Mononuclear Cells (PMN)
US Patent#7,470,722
Link to the Protocol

Telomerase Assay
Clinical Cancer Research Vol. 6, 2771-2776, July 2000
Link to the Protocol

Tetramer-binding assay
Clinical Cancer Research Vol. 12, 107-116, January 2006
Link to the Protocol

Time-Lapse Fluorescent Microscopy
Cancer Research 64, 6635-6644, September 15, 2004
Link to the Protocol

Transcription Factor Binding and Electrophoretic Mobility Shift Assays (NF-B )
Molecular Cancer Therapeutics 6, 37-50, January 1, 2007.
Link to the Protocol

Tumor Excision Assay
J Pharmacol Exp Ther Vol. 288, Issue 3, 928-937, March 1999
Link to the Protocol

Vital staining for ER distribution
Annals of Botany 93: 741-753, 2004
Link to the Protocol

VP40 Budding Assay.
PNAS December 5, 2000 vol. 97 no. 25 13871-13876
Link to the Protocol

Whole-cell HDAC enzyme assay
Clinical Cancer Research 14, 3441-3449, June 1, 2008.
Link to the Protocol

Wound-healing assay
Clin Cancer Res. 2008 Aug 15;14(16):5069-80.
Link to the Protocol

Xenograft assay
Clinical Cancer Research Vol. 11, 2670-2679, April 2005
Link to the Protocol

Xenograft tumors in vivo
Clinical Cancer Research 14, 3514-3519, June 1, 2008
Link to the Protocol

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