Neurodegenerative disease biomarker and drug discovery Protocols

Bioprotocols > Neurodegenerative disease biomarker and drug discovery

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140 items found, displaying 61 to 90.[First/Prev] 1, 2, 3, 4, 5 [Next/Last]

Assays for screening siRNA mediated inhibition of transthyretin (TTR) for treating amyloidosis : siRNA Design and Specificity Prediction and Sequence Selection, In Vitro Screening of TTR siRNAs for mRNA Suppression, In Vitro Assay of TTR siRNAs for Induction of TNF-alpha and IFN-alpha Secretion, In Vivo Reduction of Liver TTR mRNA and Plasma TTR Protein in Transgenic Mice, In Vivo Reduction of Wild-Type TTR mRNA in the Non-Human Primate Liver, In Vivo Reduction of Mutant (V30M) TTR mRNA and Protein in the Transgenic Mouse, Durability of TTR mRNA and Protein Suppression in the Transgenic Mouse, Durability of Serum TTR Protein Suppression in the Non-Human Primate, In Vivo Reduction of Mutant (V30M) TTR in Peripheral Tissues in the Transgenic Mouse, Reduction of TTR Liver mRNA by a Single Intravenous Administration in Sprague-Dawley Rats

Assays for screening ligands for alpha 7 neuronal nicotinic receptors (alpha 7 NNRs) for treating neurodegenerative diseases and schizophrenia : [3H]-Cytisine Binding Assay, [3H]-Methyllycaconitine (MLA) Binding Assay, [3H]-DPPB Binding Assay, Determination of Purity and Specific Activity

Assays for screening catechol-O-methyltransferase (COMT) inhibitors for treating Parkinson's disease (and restless legs syndrome, gastrointestinal disturbance and edema) : Assay of Mouse COMT Activity, Assay of Rat COMT Activity, Levels of L-DOPA and 3-O-methyl-L-DOPA in Plasma, Assay of L-DOPA and Catechol Derivatives, Cell Toxicity and viability Assays

Assays for screening the efficacy of combination therapy for treating neuroinflammation : Induction of Experimental Encephalomyelitis (EAE) in C57BL/6 Mice and Clinical Scoring, Acute and Chronic Drug Treatment, Detection of the Presence of Perivascular Lymphocyte Infiltrate by tissue Analysis, Clinical Scoring of the Tail Score, the Hind Limb Score, the Fore Limb Score and the Bladder Score, Body Mass Weighting

Assays for early detection of Alzheimer's disease using a blood test after an oxidative exposure of serum to assay for the presence of an elevated level of redox-reactive autoantibodies : Preparation of Serum, Treatment of Serum With and Without Hemin, Detection of Autoantibodies in the Serum (Anticardiolipin, Antiphosphatidylcholine, Antiphosphatidylethanolamine or Antiphosphatidylserine) Using ELISA

Assays for screening the efficacy of protofibril/oligomer containing N-terminal truncated Abeta as a vaccine for treating Alzheimer's disease : Preparation of Abeta3 Truncated Protofibrils/Oligomers, Synthesis of Stabilized Abeta 3 Truncated Protofibrils/Oligomers, Immunized With N-terminal Truncated Protofibrils/Oligomers in Mice and Analysis of Abeta 3 Truncated Antibodies

Assays for screening the efficacy of double-stranded ribonucleic acid (dsRNA) mediated inhibition of Huntingtin gene (HD gene) for treating Huntintin disease : Screening HD dsRNAs Against Endogenous Human HD mRNA Expression in HeLa Cells, Neuroscreen and U87MG Cells, Stability in Cerebrospinal Fluid (CSF) of Selected dsRNAs, Potency of Cholesterol-Conjugated dsRNAs Targeting HD Against Endogenous Human HD mRNA Expression in HeLa Cells, In Vivo Efficacy of Unconjugated or Cholesterol-Conjugated dsRNAs Targeting HD in Rats and Mice

Assays for screening the efficacy of artificial adeno-associated virus (AAV) mediated delivery of BACE1 targeting siRNA for improving memory or cognitive function : Development of AAV Vector Encoding for Anti-BACE1 siRNA, Effect of AAV-Mediated BACE1 Gene Silencing in the Hippocampus on Contextual Fear Conditioning in Aging Mice, Effect of AAV-Mediated BACE1 Gene Silencing in the Hippocampus in Transgenic Mouse Model of Alzheimer's Disease

Assays for screening RNA ligands (aptamers) that selectively bind to the open-channel conformation of the AMPA glutamate receptor : Aptamer Selection by SELEX, RNA Purification and Homologous Competitive Binding Assay, Transient Expression of Receptors and Whole-Cell Current Recording, Measuring the Channel-Opening Kinetics Using Laser-Pulse Photolysis Technique

Assays for analyzing flavonol glycoside that stimulate the formation of neurites and neuronal synapses in neurons and neuronal progenitor (stem) cells : Effect on length of neurite/cell using nuclear staining with DAPI, actin cytoskeleton staining with phalloidin and synaptotagmin-1 staining with antibodies, Effect on Rho inhibitors, Gene expression analysis for identifying the role of flavonol glycoside in inducing neurodifferentiation

Assays for screening calcium channel inhibitors : In Vitro Electrophysiologic Assessment of Calcium Channel Activity, In Vivo Activity Assays Using Capsaicin Induced Secondary Mechanical Hyperalgesia Model

Assays for screening the efficacy of oral administration of combination of a neurodegenerative disease drug and heterocyclic compounds of specific structures for treating cognitive impairment : Passive avoidance task test in scopolamine-induced cognitive impairment mice, Effect on extracellular levels of acetylcholine (ACh) in a rat hippocampus,

Assays for screening autophagy inducing compounds : Effect on the expression and accumulation of microtubule-associated protein light chain 3 (LC3) using cell based assay, Effect on intracellular PI(3)P levels using cell based assay, Effect on the degradation of long-lived proteins using cell based assay, Effect on cell's autophagy-related markings and LC3 II ratios, Effect on the degradation of expanded polyglutamine

Assays for screening the efficacy of intracranial transplantation of mesenchymal stem cells (MSCs) for treating Parkinson's disease : Injection of mice with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) hydrochloride and MSC transplantation, Rotarod test, Analysis of morphological changes using BrdU staining,

Assays for screening the efficacy of cerium oxide nanoparticles coated with anti-amyloid-beta antibody for treating Alzheimer's disease : Functionalization and Characterization of Cerium Oxide Nanoparticles (CNPs), Single Molecular Force Spectroscopy of CNPs, Analysis of CNPs-Ab Targeting Plaques by Immunofluorescence, Cell Viability and Death Assays, Cell Morphology Analysis Using Contrast Phase Microscopy, Analysis of Cytoskeletal Organization and the PPAR beta / delta Expression and Localization Using Immunofluorescence, Effect on Brain Derived Neurotrophic Factor (BDNF) Signal Transduction Pathway Using Immunoprecipitation

Assays for testing the efficacy of Fc region mutant antibodies with different antigen specificities for treating patients with Alzheimer's disease based ApoE genotype of the patient : Ex vivo Plaque Phagocytosis Assays to Determine Antibody Effector Function, ELISA assay for testing the ability of murine and humanized antibodies to bind C1q and induce a complement response, Contextual fear conditioning (CFC) assay in Tg2576 transgenic mice and wild-type littermate controls

Assays for screening compounds that induce Hsp70 production in cells for treating neurodegenerative diseases : Measurement of Heat Shock Protein 70 (Hsp70) Using ELISA (Hsp70 ELISA Assay), Measurement of Natural Killer Cell Cytotoxic Activity, HUVEC Cell Migration Assay, Analysis of VE-Cadherin Junctions Between HUVEC cells by Immunofluoscence, In Vivo Studies in Mouse Models (Familial Amyotrophic Lateral Sclerosis (ALS), Huntington's Disease (HD), Parkinson's Disease (PD), Alzheimer's Disease (AD) )

Assays for screening nuclear factor- kappa B (NF kappa B) inhibitors for treating neurodegenerative diseases : IkB alpha stabilization screen using a dual luciferase reporter system in the cell line OCILy3, Translocation-based assay based on high-content screen in human umbilical vein endothelial cells (HUVEC) using TNF alpha to stimulate nuclear translocation of endogenous NFkB, Structure-activity relationship (SAR) studies and characterization in physiological systems

Assays for screening BACE1 inhibitors for treating neurodegenerative diseases : High- and Medium-Throughput Screening of Small Molecule Libraries to Identify Small Molecule Modulators of BACE1 Using A Cell-Based Modified ELISA Assay for Measuring sAPP Beta, Structure-activity relationship (SAR) studies and characterization in physiological systems

Assays for screening manganese superoxide dismutase (MnSOD) modulators for treating neurodegenerative diseases : isoManganese superoxide dismutase (MnSOD) activity assay, Determination of Peroxidative Activity of MnSOD Using Luminol-Dependent Chemiluminescence Assay, Cell Based Reporter Assays for Analyzing Compounds for Toxicity, Measurement of isoMnSOD Peroxidative Activity in Explant Cultures of Cerebellum, Effect on Hydrogen Peroxide-Mediated Cell Death in Neuroendocrine Cell Line (PC12), Effect on IsoMnSOD Expression in Post-Natal Cerebellum, Effect on IsoMnSOD Expression in the Dopamine-Producing Neuronal Cell Line (PC12)

Assays for screening the efficacy of dopamine agonist (pramipexole-PPX) for treating early morning akinesia in subjects having Parkinson's disease : Effect of PPX on haloperidol-induced catalepsy, Effects of PPX on reserpine-induced early morning akinesia, Effects of PPX on extracellular dopamine levels- In Vivo Microdialysis Surgery and HPLC and LC-MS/MS Analysis of Microdialysis Samples

Assays for screening neuroprotective iron chelators : Iron-scavenging properties of the chelators in human erythroleukemia K562 cells, Neuroprotective effect on differentiation of P19 mouse embryonal carcinoma cells to neuronal cells, Neuroprotective effect on PC12 cells against serum-deprivation induced apoptosis, In vitro neuroprotective assays using rat pheochromocytoma type 12 (PC12) cells and human neuroblastoma SH-SY5Y cells, Effect rat brain monoamine oxidase (MAO) activity, Effect on lipid peroxidation in brain mitochondrial fraction, Effect on topical photoprotection in guinea pigs, Lipophilicity testing for analyzing transport property of the chelators, In vivo test using animal model of Parkinson's disease

Assays for the identifying inhibitors of lipoxygenase enzymes for treating neurodegenerative diseases : Antioxidant Activities of the Compounds, Effect on Oxidative Glutamate Toxicity in Neuron-Like Mouse Hippocampal Cell Line (HT22), Effect on Oxidative Stress Using Rat Primary Cortical Neurons, Neuroprotective Effect on Oligodendroglial Cells

Assays for screening sirtuin modulating compounds : Idenitification of SIRT3 modulators using fluorescence polarization assay, Cell based fat mobilization assay using 3T3 L1 cells for sirtuin activity, ATP cell-based assay using NCI-H358 cells for testing the effect of the SIRT1 activator

Assays for screening inhibitors of BACE-1 : BACE-1 Cloning, Protein Expression and Purification, Peptide Hydrolysis Assay Using EuK-biotin labeled APPsw Substrate

Assays for screening compounds that potentiate AMPA receptors (AMPAR) : Calcium influx fluorescence assay using HEK 293 cells stably expressing human GluR2 flip (unedited) AMPA receptor subunit, Whole cell voltage-clamp electrophysiology assay for measuring AMPA-evoked current recorded from rat cultured hippocampal neurons, Electrophysiological activity at human recombinant GluR21 homomeric AMPARs

Assays for screening humanin receptor or humanin-like polypeptide receptor (HNR) agonists and antagonists for treating neurodegenerative diseases : Primary Cortical Neurons And Cell Viability Assays, Immunofluorescence-Based Binding Assay for Detecting the Association Between HN and the Endogenous HN receptor, Pull-Down Assays Using F11 cells

Assays for screening compounds for treating activity of cognitive dysfunction disease : Inhibition test of beta amyloid aggregation, Inhibition test of beta amyloid aggregation lysis, Inhibition test of beta amyloid toxicity, Determination of cytotoxicity, Alzheimer's disease acute model experiment-Y maze test, Alzheimer's disease acute model experiment- passive avoidance test, Water maze test, Cognitive capacity test, Immunochemistry staining of Alzheimer's disease acute model mouse recovering cognitive capacity

Assays for screening the efficacy of marine phycotoxin gymnodimine for treating Alzheimer's disease : Determination of the cellular viability of treatment with gymnodimine, Effect of gymnodimine on overexpression of intracellular beta-amyloid and tau hyperphosphorylation, Effect of gymnodimine on overexpression of intracellular beta-amyloid and tau hyperphosphorylation by Western blot analysis, Effect of the compounds of the present invention on basal phosphorylation of tau in control neurons by Western blot analysis

Assays for screening the efficacy of glial cell derived neurotrophic factor (GDNF) targeted anitsense oligonucleotides for treating Parkinsonism : Delivery of Oligonucleotides Specific for GDNF Antisense Transcripts into Primates, Effect of GDNF Antisense Oligonucleotides on Monkey Model for Parkinsonism

140 items found, displaying 61 to 90.[First/Prev] 1, 2, 3, 4, 5 [Next/Last]