Tumor and tumor cell assay Protocols

Bioprotocols > Tumor and tumor cell assay

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Adipose triglyceride lipase (ATGL) assay

Analysis of melanoma-endothelial cell communication using in vitro 2-dimensional coculture systems

Analysis of brain tumor stem cells (BTSC) in an immunocompetent mouse brain tumor model : Generation of neurospheres, Intracranial brain tumor model, Analysis of the expression of CD133, Oct4, Nanog, Nestin in CT-2A cells and neurospheres using immunofluorescence and flow cytometry, In vitro proliferative and invasive properties of CT-2A cells and neurospheres

Analysis of differentiation potential of blood monocytes in tumor-bearing mice (TBMs)

Analysis of phagocytosis of apoptotic K562 leukemia cells by immature monocyte-derived dendritic cells (iMoDCs) and peripheral blood dendritic cells (PBDCs) and identification of cell-surface molecules potentially involved in uptake of apoptotic and necrotic K562 leukemia cells by CD11c DC

Analysis of surface antigens associated with differentiation and function on the CD14+CD16+ monocytes by flow cytometry

Analysis of the phenotype and antigen uptake of dendritic cell (DC) and HLA-DR+ immature cells (DR+IC) in peripheral blood mononuclear cells (PBMC) using four-color flow cytometry

Analysis of treatment response-related genes in patients with locally advanced non-small cell lung cancer using mutant-enriched polymerase chain reaction (ME-PCR; liquidchip technology)

Analysis of catena (serosal cancer stem cells) surface phenotype : FACS surfacesome analysis, CD gene expression analysis, Catena-specific gene expression corresponding to human surfaceome gene

Analysis of melotoxicity of chemotherapeutic drugs by cell surface antigen analysis

Analysis of the expression pattern of COX-2 immunoreactive protein in bladder carcinomas using immunohistochemistry

Analysis of the expression of COX-2 in tumor tissues (e.g. urinary bladder cancer)

Anchorage-independent assays (BxPC-3 and PANC-1)

Anti-metastatic Drug Efficacy Screening Models : Orthotopic mouse model of uveal melanoma micrometastasis, SCCHN metastasis model

Antioxidant Activity Assays : 1,1-Diphenyl-2-picrylhydrazyl (DPPH) Radical Scavenging Activity, ORAC Antioxidant Activity Assay, Ferric Reducing/Antioxidant Power (FRAP) Assay

Apoptosis assays : Flow cytometric analysis of Annexin V and 7-amino-actinomycin D (7-AAD) staining and Flow cytometric analysis of activated caspases

Approaches for focused library screening of compounds for identifying kinase inhibitors (e.g. PRK1 inhibitors) : Focused Library Screening, Homology Modeling, Kinase Inhibitor Screening Using LanthaScreen TM Eu Kinase Binding Assay Kit, Cellular Testing Using RT-qPCR and Western blotting

Assay for measuring DNA strand breaks using fluorimetric detection of alkaline DNA unwinding (FADU)

Assay for screening inhibitors of adipose triglyceride lipase (ATGL) for treating cachexia

Assaying thecapacity of dendritic cell (DC) and HLA-DR+ immature cells (DR+IC) to stimulate allogeneic T-cell proliferation using mixed lymphocyte reaction (MLR)

Assays and methods for identifying methylation specific biomarkers for the diagnosis of cancer (e.g. colorectal cancer) : Extraction of DNA from Surgically Resected and Frozen Specimen, Preparation of Control Sample, Treatment of DNA with Sodium Hydrogen Sulfite, Quantitative Analysis of Methylation

Assays and methods for identifying methylation specific cancer biomarkers (e.g. bladder cancer) : DNA Isolation from Patient Samples, Differential Methylation Hybridization (DMH) and Data Analysis, Illumina Custom Golden Gate Methylation Assay and Statistical Analysis, Validation of Methylation of Individual CGIs

Assays and methods for the identification of methylation specific cancer drug targets and anticancer drugs (e.g. breast cancer) : Effects of drug on cell growth of cancer cell lines using cell growth and apoptosis (cell cycle analysis and DNA fragmentation) assays, Identification of molecular markers associated with drug response using gene and protein expression analysis, Effect of siRNA mediated silencing of selected markers, Analysis of epigenetic modification of markers using DNA demethylating agent, methylation specific PCR (MSP) and Chromatin immunoprecipitation Q-PCR (CHIP Q-PCR)

Assays and methods for identifying genotoxic effect of chemical carcinogens on DNA methylation : Chemicals, Viability Assays and Dose Selection, DNA Extraction and Enzymatic Hydrolysis of DNA, Global DNA methylation analysis by quantitative profiling of 5-methyl-2'-deoxycytidine using liquid chromatography-mass spectrometry (LC-ESI-MS/MS)

Assays and methods for the identification of epigenetic biomarkers of cancer (e.g. esophageal adenocarcinoma) : DNA and RNA Extraction From Tissue Samples, Bisulfite Treatment and Real-Time Methylation-Specific PCR, Real-Time Quantitiative RT-PCR, 5-Aza-dC Treatment of Esophageal Cancer Cell Lines, Data Analysis and Statistics

Assays and methods for studying the role of long non-coding RNAs (lincRNAs; e.g.HOTAIR lincRNA ) in modulating the cancer epigenome : RNA isolation from normal breast organoid and metastatic breast carcinoma samples, HOTAIR LincRNA expression and survival/metastasis analysis of primary breast tumors, RNA expression analysis using qRT-PCR, Microarray analysis, Non-radioactive in situ hybridization of paraffin sections, RNA interference for HOTAIR, Cell proliferation assays, Mammary fat pad xenografts, Mouse tail-vein assay, In vivo bioluminescence imaging (mouse), TaqMan real-time PCR assays, Gene set analysis

Assays and methos for evaluating the efficacy of combination of HDAC inhibitor (HDACi) and aromatase Inhibitor for treating cancer : Cell Migration Assay, of HDACi Mediated Sensitization of ER-Negative Breast Cancer Cells to Aromatase Inhibitors, Radiometric 3H2O Release Assay for Aromatase Activity , Studies on ER Negative Breast Cancer Xenograft Model, Human Clinical Trial of the Safety and Efficacy of Combination of HDAC Inhibitor and Aromatase Inhibitor

Assays and methods for studying transduction signaling pathways through which Bisphenol-A (BPA) influences cell proliferation and migration in breast cancer cells and cancer-associated fibroblasts : Model system- SKBR3 breast cancer cells and CAFs that lack the classical ERs, Luciferase reporter assays (Ctgf, c-FOS and EGR-1) Reverse transcription and real-time PCR, Gene silencing experiments, Wound-healing assay, Proliferation assay SKBR3 cells and CAFs, Statistical analysis using analysis of variance followed by Newman-Keuls testing

Assays and methods for identifying the role of proteins expression (e.g.OPRT) in the sensitivity of head and neck squamous cell carcinoma (HNSCC) to 5-Fluorouracil (5-FU) chemotherapy : Human Head and Neck Squamous Cell Carcinoma Cell Culture, Vector Construction and Transfection, Western Blot Analysis of Cultured Cells, In Vitro Proliferation Assays, Drug Sensitivity Assay, Statistical Analyses Using the Mann-Whitney U test

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