Tumor and tumor cell assay Protocols

Bioprotocols > Tumor and tumor cell assay

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580 items found, displaying 31 to 60.[First/Prev] 1, 2, 3, 4, 5, 6, 7, 8 [Next/Last]

Assays and methods for testing the efficacy of anti- matrix metalloproteinase 14 (anti-MMP-14) antibodies for treating cancer : Selection and Screening of Anti-MMP-14 Fabs and IgGs, Pre Screening Phage ELISA, DNA and Amino Acid Sequences of MMP-14 Binding Fabs that Inhibit MMP-14, IC50 Values for MMP-14 Inhibition of MMP-141 Binding Fabs and IgGs, Ki values for MMP-14 Inhibition by Anti-MMP-14 IgGs, Cross-reactivity of MMP-14 Binding IgGs and Fabs Against Other MMPs and TACE , Cross-Reactivity of MMP-14 Binding Fabs and IgGs with MMP-16, Cross-Reactivity of MMP-14 Binding Fabs with MMP-16 and MMP-24, Binding of MMP-14 IgGs to Tumor Cells Expressing MMP-14, Inhibition of Pro-MMP-2 Activation by MMP-14 by MMP-14 Binding IgGs, Germlining, Inhibition of Tube Formation by MMP-14 Binding IgGs, Inhibition of MDA-MB-231 Tumor Growth and Metastasis by MMP-14 Binding IgGs, Inhibition of MDA-MB-435 Breast Tumor Growth by MMP-14 Binding IgGs, Inhibition of B16 Melanoma Tumor Growth by MMP-14 Binding Antibodies, Inhibition of Prostate-Tumor Growth by MMP-14 Binding Antibodies, Inhibition of BT747 Breast Tumor Growth by MMP-14 Binding Antibodies, MMP-14 in Mouse Models Of Arthritis,

Assays and methods for testing the efficacy of anti-CD74 immunoliposomes (immunoconjugates) for treating cancer : Preparation of Anti-CD74 Immunoliposomes Carrying FUdR-dO, Cellular Uptake and Metabolism of the Anti-CD74 Immunoliposomes, Specificity of Immunoconjugates, Endocytosis of HPTS-Containing Immunoliposomes, Cytotoxic Activity Assay of Immunoliposomes Using Raji lymphoma cells, Therapy of Chronic Lymphocytic Leukemia with Anti-CD74 immunoliposomes (Patients cell separation and culture, Flow cytometry assays, Immunoblot analysis, Preparation of Immunoliposome, Statistical analysis)

Assays and methods for identifying potential new targets of polyphenol piceatannol, a tyrosine and serine/threonine kinase inhibitor : Cell Culture, Colony Formation, and Proliferation Assays Using Human LNCaP, DU145, and PC-3 cells, Effect of Piceatannol on Cell Cycle Analysis, Preparation of Whole-Cell Extracts and Western Blot Analysis

Assays and methods for developing prodrugs (oral form) DNA methyltransferase (DNMT) inhibitor, Azacitidine and decitabine : Monitoring the In vivo absorption, Distribution, and Metabolism Using LC-MS/MS Analysis, Determination of Drug Stability in pH1 (2% Phosphoric acid), pH3 (0.1% Formic Acid), pH5 (K3PO4), and pH7.4 (Dulbeccos PBS), Log P Determination Using OSIRIS Property Explorer software, Permeability Studies Using Caco-2 Monolayers, Pharmacokinetics and Metabolism Studies in Mice, Global Methylation Detection Assay Using EpiGenteks Methylamp Global DNA Methylation Quantification Kit, p15INK4B Methylation Analysis, In Vivo Antileukemic Activity Using L1210 lymphocytic leukemia model in C57BL/6 × DBA/2 F1(BDF1) female mice, In Vivo Toxicity Studies in CD-1 mice

Assays and methods for screening the efficacy of anti-AXL (Ark, UFO, Tyro-7) receptor tyrosine kinase antibodies for treating drug resistant cancer : Generation and Chimerization of Rat Anti-AXL Monoclonal Antibodies, Effect of Rat Anti-AXL Antibodies on Human Prostate Carcinoma Growth and Metastasis in Nude Mice, Design and Generation and Production of Humanized Antibody Gene of Rat Anti-Human AXL Monoclonal Antibody, Evaluation of Affinity of Humanized Antibody of Rat Anti-Human AXL Monoclonal Antibody for Human AXL-Fc Fusion Protein, Absorption Coefficient Calculation and Direct Protein Absorbance Assay of Humanized Antibody of Rat Anti-Human AXL Monoclonal Antibodies, Determination of Binding Site (Epitope) for the Rat Anti-Human AXL Monoclonal Antibody, Effect of Humanized Anti-AXL Antibodies on Ligand-Induced AXL Phosphorylation In Vitro Using ELISA and Western-Blot Analysis, Effect of Humanized Anti-AXL Antibodies on Ligand-Induced Phosphorylation of AXL Downstream Signaling Molecules In Vitro, Effect of Humanized Anti-Axl Antibodies on Ligand-Induced Phosphorylation of ERK1/2 and AKT and GSK-313 and TSC2 and mTOR and S6K1 In Vitro, Effect of Humanized Anti-Axl Antibodies on Induction of Axl Receptor Internalization, Measurement of Thermal Stability of Humanized Antibody Using Differential Scanning Calorimetry (DSC)

Assays for identifying determinants of acquired resistance to BRAF inhibitors : Cell lines, DNA analysis, Western blot analysis, Cell growth inhibition and apoptosis assays, Affymetrix expression analyses, GSK1120212 MEK1 model construction

Assays for studying the effect of dual PI3K/mTOR (both mTORC1 and mTORC2) inhibitors : Clonogenic survival assay, Analysis of protein phosphorylation, Analysis of gammaH2AX foci, Cell cycle assay, Analysis of apoptosis, Capillary tube formation and endothelial cell migration, Statistical analyses

Assays for studying the effects of high-risk human papillomavirus (HPV) infection and folic acid (FA) supplementation in oral cancer cell lines : Human OSCC cell culture, Proliferation assays, Survival and viability Assays, DNA isolation, PCR, and RFLP, Determination of the cellular phenotype of p53 mRNA transcription and confirmation of HPVspecific mRNA production post-transfection

Assays for evaluating the effects of DNA methyltransferase (DNMT) inhibitors on the radiosensitivity of human cancer cell lines : Cell culture and DNMT inhibitors, Clonogenic assay, Flow cytometric analysis, Western blot for DNMT1, DNMT3A/3B, and cleaved caspase-3, Immunocytochemistry

Assays for determining the effects of Norcantharidin (NCTD) on hepatocellular carcinoma (HCC) cell metastasis : HCC (Huh7) cell culture, Determination of cell viability (MTT assay), In vitro wound closure, Cell invasion and migration assays, Determination of MMP-9 and u-PA by zymography, RNA preparation and TaqMan quantitative real-time PCR, Preparation of total cell lysates and nuclear fraction, Transfection and MMP-9 or u-PA promoter-driven luciferase assays, Chromatin immunoprecipitation analysis (ChIP)

Assays for analyzing the function of orphan nuclear receptor liver receptor homolog 1 (LRH-1, NR5A2) in breast cancer cells : MCF-7 cell culture, RNA extraction and quantitative real time PCR (qPCR), Western blot analysis, Chromatin immunopreciptiation, Electrophoretic mobility shift assays (EMSA), Luciferase reporter assays, Cell proliferation assay

Assays for analyzing the effect of phenoxodiol on cell death pathways on the prostate cell lines : Cell proliferation assay, FACS analysis, Caspase inhibition assay, Detection of the mitochondrial potential sensor JC-1, Reverse transcription and quantitative RT-PCR,

Assays for identifying the potenital chemopreventive and chemotherapeutic targets of curcumin (diferuloylmethane) : Cell cycle dependent cell death induced by curcumin, Binding of curcumin to Cdc27/APC3 subunit of APC/C, Cdc27 phosphorylation by curcumin, Inhibition of APC activity by curcumin

Assays for studying the regulatory mechanisms through which apoptosis and autophagy were modulated in tumor cells treated with Akt inhibitor MK-2206 : siRNA transfection, Measurement of autophagy, Western blot analysis, Apoptosis assays, Measurement of mitochondrial membrane potential, Detection of ROS, Cellular viability assay and Animal study

Assays for determining the potential therapeutic utility of HDACi differentiation therapy for NUT midline carcinoma (NMC) : Histone deacetylase inhibitors (HDACi) treatment of NMC to resotre bulk chromatin acetylation that closely mimics the effects of siRNA-mediated attenuation of BRD4-NUT expression (In vivo studies , Quantitative reverse transcriptase polymerase chain reaction, Chromatin immunoprecipitation quantitative polymerase change reaction (ChIP-qPCR))

Assays for Testing Antagonists of Hedgehog Signaling Pathway : Cloning of Smoothened (Smo) and Generation of Stable Recombinant Smo Expressing Cell Lines, Testing the interaction of compounds with the Smo receptor by displacement binding assay using fluorescent ligand for the Smo receptor (Bodipy-Cyclopamine), Sonic (Shh) Dependent Alkaline Phosphatase Assay

Assays for Screening EP4 Receptor Selective Antagonists : Experiments using xenograft animal models of human prostate cancer, breast cancer and lung cancer

Assays for Screening Anti-Hsp70 antibodies : Purification of Antibodies by Thiophilic Chromatography, Characterization of Binding Properties and Biological Activity of Antibodies, Effects of Antibodies on CT26 Tumor Cells In Vitro, Intraoperative In Vivo Imaging of Hsp70 in Tumor-Bearing Mice, Near-Infrared Fluorescence (NIRF) In Vivo Imaging of Hsp70 in Tumor-Bearing Mice, Monoclonal Antibody cmHsp70.1 Initiates ADCC in Membrane Hsp70+ Tumors In Vitro

Assays for identifying inhibitors of the TRIM62 protein : Inactivation of TRIM62 Enhances Effects of ErbB2 Antibodies on p27 Expression in ErbB2 Amplified Breast Cancer Cell, Assessment of the Effect of TRIM62siRNA on Normal Breast Cells and HER2 Positive Breast Cancer Cells

Assays for Screening Histone Deacetylase Inhibitors : A2780 tumor cell colorimetric assay for cell toxicity or survival, In vitro assay for inhibition of histone deacetylase, Determination of antiproliferative activity on A2780 cell, Kinetic solubility in aqueous media, Parallel artificial membrane permeability analysis, Metabolic stability, p21 induction capacity, p450 inhibiting capacity, A 2780 mouse xenograft model

Assays for Identifying Compounds for the Treatment of Skin Cancer : Effects of Compounds on Progression of Subcutaneous Gliomas Already Established in Rats, Effects of Compounds on the Progression of Subcutaneous Tumors in a Prostate Cancer Cell Implantation Model in Athymic Mice, Inhibition of the Mitogenesis of Fibroblasts Induced by the Fibroblast Growth Factor-1 (FGF-1), Analysis of the Structural Interaction of Compounds with the Fibroblast Growth Factor-1 (FGF-1)

Assays for screening compounds for the treatment of hormone-dependent cancers or for tumors expressing androgen receptors : Anti-Proliferative Activity on LNCaP in Complete Medium, Anti-proliferative activity of the compounds on LNCaP in Medium Without Steroids, Antiproliferative Activity on DU-145 in Complete Medium, Measurement of the Disappearance of the Androgen Receptor

Assays for Screening Compounds for the Treatment of Cancers : In Vitro Effects on a Panel Of Tumor Derived Cell Lines, In Vitro Effects on a Panel of Primary Human Tumor Cells, Antitumor Effect in The Human Breast Cancer Model BT-474, Antitumor Effect in the Rat Breast Cancer Model BN-472 and Antitumor Effect in the Rat Pancreatic Cancer Model CA20948

Assays for Screening P2X3 AND P2X 2/3 Antagonists for the Treatment of Cancer Bone Pain : P2X3/P2X2/3 FLIPR (Fluorometric Imaging Plate Reader) Assay, Cancer Bone Pain in C3H/HeJ Mice

Assays for Screening Insulin-Like Growth Factor 1 Receptor (IGF1R) Inhibitors : Inhibition of IGF1R-Catalyzed Substrate Phosphorylation, Inhibition of IGF1-Induced Signaling and Other Signaling Pathways (e.g. the EGF, PDGF and Insulin-Induced Signaling) in Intact Cells, Proliferation Assay, Clonogenic Assay, In-Vivo Effects on Prostate, Ovarian, Melanoma and Pancreatic Tumor Growth in Xenograft Models, In-Vitro Inhibition of Keratinocyte Growth

Assays for Screening Purine Derivative for the Treatment of Cancers : Antitumor Activity Against Various Tumor Cells (Adhesive Cancer Cells and P388 Cells (Hematolotic Tumor)), Antitumor Activity Against Breast Cancer Cells, Effects of Compounds on Nude Mice Transplanted with Human Lung Cancer A549, Effect of Compounds on Nude Mice Transplanted with Human Prostate Cancer PC-3 and Effect of Compounds on Nude Mice Transplanted with Human Colon Cancer WiDr

Assays for Screening Receptor Protein Tyrosine Kinase AXL Inhibitors : Phospho-AKT in-Cell Western Assay

Assays for Screening PI3 Kinase Inhibitors : Assays to determine the IC50 of compounds, HDAC inhibitory Activity Using the Biomol Color de Lys system, In Vitro Assay to Test the Ability of Compounds to Inhibit mTor Serine/Threonine Protein Kinase, Cell Proliferation Assay,

Assays for Determining the Inhibitory Activity of Compounds Against Kinases : Assay for determination of inhibition of kinases under physiological conditions (pH 7.4) and Cellular calcium flux assays

Assays for Screening Novel Proteasome Inhibitors : Development H1299-PIR Reporter Cell Line and its Application for Image-Based, High-Throughput Screening Assay for Proteasome Inhibitors, Screening for Novel Proteasome Inhibitors in the Diversity Set of the NIH/NCI Chemical Library, Biochemical Validation of the Inhibitory Effects of the Hit Compounds, Effect of the Novel Proteasome Inhibitors on Cell Viability and Characterization of the Possible Mode of Action of the Proteasome Inhibitor NSC321206, Discovered Using the PIR System

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