Tumor and tumor cell assay Protocols

Bioprotocols > Tumor and tumor cell assay

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580 items found, displaying 211 to 240.[First/Prev] 4, 5, 6, 7, 8, 9, 10, 11 [Next/Last]

Assays for screening the inhibitors of cMET kinase : Determination of inhibition of cMET by the use of capillary electrophoresis with fluorescence quantification of a phosphorylated peptide product for cMET, Determination of inhibition of cell viability

Assays for screening the efficacy of siRNA mediated inhibition of transmembrane phosphatase with tensin homology (TPTE) for anti-cancer drug development : Designing of siRNA Duplexes, In Vitro Phosphatase Assay of TPTE, Cell migration assays, Cell Proliferation Assay Using DELFIA Cell Proliferation Kit, Cell Cycle Analysis and Apoptosis Assay, Calcium Mobilization Assay Using FLIPR Calcium 3 Assay Kit, In Vivo Tumor Growth Analysis and Experimental Metastasis Assay, Statistical Analysis of TPTE Expression in Tumors in Relation to the Metastatic Rate of the Patients

Assays for screening the efficacy of microglial precursor cells for treating glioma : Generation of microglial precursors from mouse embryonic stem cells (ESdM), Induction of experimental gliomas in a syngenic animal model to avoid immune reaction against allo-antigens expressed by the tumor, Intratumoral injection of microglial precursors into experimental gliomas, In vitro experiments with murine ESdM and glioma

Assays for analyzing Gr-1+ monocytes as therapuetic drug targets for cancer immunotherapy : Characterization of blood Gr-1+ monocytes, Analysis of in vivo potential of Gr-1+ monocytes to differentiate into DCs in tumor tissues, Characterization and cytokine secretion of Gr-1+ mo-DCs, In vivo and in vitroTreg proliferation of Gr-1+ mo-DCs induced CD4+CD25+, Effect of intratumoral delivery of IL-10 soluble receptor and IL-3 genes in growth of C26 tumors

Assays for validating the use of carcinoma homing peptide (CHP) for targeted delivery of gene products to tumors

Assays for screening a compounds that inhibit the binding between stromal cell-derived factor 1 (SDF-1) and C-X-C chemokine receptor type 4 (CXCR4) : Study for inhibition of binding human SDF-1 to CEM cells, Measurement of the influence of a compounds on blood pressure and heart rate, Measurement of maximum plasma level (CMax) and bioavailability (BA), Phospholipid accumulation measurement, Analysis of artificial lipid membrane binding by using Biacore (RTM) S51 system

Assays for screening the efficacy of OCT4 derived peptides as universal cancer vaccine : Synthesis of OCT4 peptide library, Detection for OCT4 reactive T cells in fresh peripheral blood mononuclear cells (PBMCs), CFSE proliferation assay, Dendritic cell generation and expansion of OCT4 specific T cells, Flow cytometry for the detection of intracellular cytokines

Assays for identifying CD166 can be used as a potential surface marker for identifying castration resistant prostate cancer (CRPC) cells for targeted drug delivery

Assays for screening the efficacy of combination therapy using gemcitabine with a checkpoint kinase 1 (Chk1) inhibitors for targeting pancreatic cancer stem cells

Assays for screening the efficacy of beta-arrestin2 as a molecular target in chronic myelogenous leukemia (CML)

Assays for characterizing chemosensitivity of glioblastoma (GBM) stem cells : Isolation of cancer stem cell (CSC) from slow proliferating glioblastoma cell line U138MG, Generation of GBM neurospheres and doxorubicin treatment, Cell proliferation assay, Colony formation unit assay, Secondary sphere formation assay, Analysis of multi drug resistant (MDR) gene expression markers after drug treatment using RT-PCR, Analysis of MDR protein p-glycoprotein by Western blotting

Assays for screening the efficacy of goodpasture antigen-binding protein-1 (GPBP-1) inhibitors as drugs for the treatment of doxorubicin-induced pulmonary fibrosis

Assays for studying the effect of Des A fibrin on migration of malignant tumor cells

Assays for identifying gene signatures associated with adjuvant chemotherapy response in non-small cell lung cancer (NSCLC) patients : RNA Isolation and Microarray Profiling, Microarray Data Analysis and Gene Annotation, Derivation of Signature, Validation in Separate Microarray Datasets, Statistical Analysis

Assays for identifying biomarkers for detecting ovarian cancer : Isolation and profiling of ovarian cyst fluid samples, High throughput sample fractionation by tandem equalizer bead and mercapto ethyl pyridine (MEP) beads, High throughput sample fractionation by tandem antibody libraries beads,

Assays for identifying liver cancer biomarker : Collection of clinical tissues and plasma, Separation of glycoproteins in non-timorous and timorous liver tissue, 2-Dimensional fluorescence differential gel electrophoresis (2-D DIGE), Identification of 2-D DIGE-separated proteins, Validation of HCC biomarker expression levels in tissues by Western blot analysis and immunohistochemical staining, Validation of HCC biomarker protein secretion level in human plasma by immunoprecipitation and Western blot analysis, Validation of biomarker protein in human plasma by Nano-LC-MS/MS system

Assays for screening anti-cancer effects of anti-neuropilin-1 (NRP1) antibodies : Cell culture, DRG and hippocampal collapse assays, Cell migration assays using a modified Boyden chamber assay, Bead outgrowth assay using HUVEC-coated beads, FACS analysis using HUVECs, Cell adhesion assay, Mouse neonatal retinal vascular assay, Mouse skin vessel permeability assay, Cell proliferation assay, VEGFR2 phosphorylation assay

Assays for testing the role of inflammation-mediated stem cell proliferation/differentiation in urinary bladder carcinogenesis : Immunohistochemical analysis of the expression of stemness markers (Oct3/4 and CD44v6) in urinary bladder tissues, Analysis of the expression and nuclear localization of COX-2 in cancer tissues

Assays screening antibody based disrupting of functional leucine-rich repeat-containing G protein-coupled receptor (LGR) signaling and inhibiting growth solid tumor cancer stem cells : LGR5 Is Over-Expressed In Cancer Stem Cells Relative to Non-Tumorigenic Tumor Cells , Generation of Anti-RSPO1 or Anti-LGR5 Antibodies, In Vivo Prevention of Tumor Growth by Targeting RSPO1 and LGR5

Assays Supporting the Oxidative Cellular Energy Production Process and Mitochondrial Activity of Cancer-Initiating Cells (Glioma-Initiating Cells)

Assays to characterize the anti-tumor activity of Hh pathway inhibitors using in vivo pre-clinical model of serous ovarian cancer : Immunohistochemical analysis using an ovarian cancer tissue microarray, Tumor collection and propagation in vivo, Treatment of mice with Hh pathway inhibitors (Cyclopamine and IPI-926), RT-PCR analysis, Analysis of Gli1 expression in human ovarian tumor stroma and Statistical Analysis

Assays to test whether leukemic stem cells in CML use the beta-catenin pathway for self-renewal : Bone Marrow and Peripheral Blood Samples, Isolation of Hematopoietic Stem Cell and Myeloid Progenitor Populations, Colony Forming Cell Assays, Quantitative Reverse Transcriptase-PCR for BCR-ABL,beta-catenin and LEF-1 expression, Beta-catenin FACS Analysis, Confocal Fluorescence Microscopy, Lentiviral Transduction with LEF/TCF-GFP, PGK-.beta.-catenin-IRES-GFP or PGK-Axin-IRES-GFP

Assays to identify IkB kinase1 (IKK1) as a potential therapeutic drug target of prostate cancer (PCa) : Effect of Inhibition of IKB Kinases on Cell Growth and AR Signaling in PCa Cell Lines, Effects of siRNA mediated Knockdown of IKK1 or IKK2 on PCa Cell Growth, Hormone-Induced Nuclear Translocation of AR by IKK1, Effects of IKK1 siRNA on the expression of AR target genes. Immunoprecipitation and Kinase Assay to Identify AR as a IKK1 Target

Assays to study the role of CD133+ tumor stem-like cells (TSCs) in glioblastoma (GBM) radioresistance : GBM TSC Culture (neurosphere-forming cultures NSC11), Generation of IC Xenografts, Irradiation of CD133+ TSCs, Immunofluorescent Analyses of gamma H2AX and 53BP1 Foci, Generation of Luciferase-Expressing Cells and Bioluminescence Imaging, Determination of Percentage of CD133+ Cells in IC Xenograft Tumors

Assays to evaluate the that elevated levels of blood CD14+CD16+ monocytes are associated with poor prognosis in patients with cholangiocarcinoma (CCA)

Automated quantitative whole animal bio-imaging assay for first-line in vivo screening step in the anti-cancer drug target discovery pipeline

Automated noninvasive quantitative whole animal bioimaging method for dissemination of xenotransplanted human cancer cells in zebra fish embryos in 96-well format

Automated spheroid model of Capan-2 pancreatic cancer cell spheroids in 96 well-plates

Automated immunoassay (hybridoma screening) using plasma samples for characterizing anti-protein induced by vitamin K absence (PIVKA-II) monoclonal antibodies for treating hepatocellular carcinoma (HCC) and liver cancer : Preparation of acridinium conjugates of monoclonal antibody, Architect immunoassay using the 4 antibodies coated on the magnetic microparticles, Evaluation of Assay Performance

Bioassay-Guided Fractionation, Isolation, and Identification of the Active Principle : Antioxidant activity of the extract of Turbinaria ornata

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