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We have a unique collection of protocols from journals and patents. We have listed thousands of protocols and in most cases, the titles of protocols listed in our site are similar to the "Materials & Methods" section of the journals. Sometimes, it is not easy to categorize these protocols. We are in the process of categorizing these protocols. Meantime, we strongly recommend you to search for the protocols using the search function.
Bioprotocols > siRNA/RNAi

siRNA/RNAi Protocols

The protocols listed in this section are extracted from scientific journals or patents or laboratory websites. We have cited only the journals that are freely available to view. Please be informed that the free services offered by listed journals may not be available globally. If you find difficulty in viewing the journals listed in our site, please let us know.
ADK-Immunocytochemistry
Exp Neurol. 2007 November; 208(1): 26-37.
Link to the Protocol

Assay for adenosine
Exp Neurol. 2007 November; 208(1): 26-37.
Link to the Protocol

Asymmetric requirement of ribonucleotides in siRNA for effective gene silencing
Nucleic Acids Res. 2008 April; 36(7): 2136-2151
Link to the Protocol

Cell culture and gene-silencing activity assay (mouse embryonic stem cells)
Nucleic Acids Res. 2008 April; 36(7): 2136-2151
Link to the Protocol

Chip hybridisations and analysis of whole-genome expression data
BMC Dev Biol. 2007; 7: 46.
Link to the Protocol

Construction of psiCHECK derivatives and the assay of seed-sequence- and passenger-strand-based off-target activity
Nucleic Acids Res. 2008 April; 36(7): 2136-2151
Link to the Protocol

Development of a recessive genetic screen for RNAi mutants in Blm-deficient ES cells
Nucleic Acids Res. 2009 March; 37(4): e34
Link to the Protocol

Dicer assay
Science 26 September 2003: 1921
Link to the Protocol

dsRNA synthesis and microinjections
Dis Model Mech. 2008 Sep-Oct; 1(2-3): 131-143
Link to the Protocol

ES-to-differentiated cell fate transition induced by functional DNA-modified siRNA-dependent inactivation of Oct4
Nucleic Acids Res. 2008 April; 36(7): 2136-2151
Link to the Protocol

Establishment of inducible RNAi in embryonic stem cells
Proc Natl Acad Sci U S A. 2007 Dec 26;104(52):20850-5
Link to the Protocol

Establishment of the SDVFi RNAi System in ES Cells
Proc Natl Acad Sci U S A. 2007 December 26; 104(52): 20850-20855
Link to the Protocol

Gel shift assay (siRNA)
Science 26 September 2003: 1921
Link to the Protocol

Generation of Rest mutant ES cells
PLoS ONE. 2008; 3(11): e3656.
Link to the Protocol

Generation of lentiviral constructs for the expression of anti-ADK miRNAs
Exp Neurol. 2007 November; 208(1): 26-37.
Link to the Protocol

Generation of stable knock-down and rescue cell lines
PLoS Pathog. 2009 August; 5(8): e1000546
Link to the Protocol

Genome-wide siRNA screen
Genes Dev. 2009 April 1; 23(7): 837-848
Link to the Protocol

Histological and KA-induced cell death analysis
Exp Neurol. 2007 November; 208(1): 26-37.
Link to the Protocol

Human Genome siRNA Libraries
PLoS One. 2009 Oct 5;4(10):e7339
Link to the Protocol

Isolation, lentiviral transduction and culture of hematopoietic stem cells
Acta Biochim Biophys Sin 2008, 40: 711-720
Link to the Protocol

Knockdown of ADK in hMSCs by lentiviral RNAi
Exp Neurol. 2007 November; 208(1): 26-37.
Link to the Protocol

Lentiviral constructs and lentivirus preparation
Acta Biochim Biophys Sin 2008, 40: 711-720
Link to the Protocol

Lentiviral Transduction of HSF-6 Cells with eGFP Transgene
Experimental Biology and Medicine 232:1368-1380 (2007)
Link to the Protocol

Lentiviral transduction of hMSCs
Exp Neurol. 2007 November; 208(1): 26-37.
Link to the Protocol

microRNA-based conditional RNA interference
Proc Natl Acad Sci U S A. 2006 September 12; 103(37): 13759-13764
Link to the Protocol

Preparation of recombinant DCR-2 and R2D2 protein
Science 26 September 2003: 1921
Link to the Protocol

Production of lentiviral vectors
Exp Neurol. 2007 November; 208(1): 26-37.
Link to the Protocol

Purification of the siRNA-generating activity
Science 26 September 2003: 1921
Link to the Protocol

Quantitative analysis of ADK knockdown by Western blot analysis
Exp Neurol. 2007 November; 208(1): 26-37.
Link to the Protocol

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications
Epigenome NoE
Link to the Protocol

RNAi and Viral Vectors (Human Bone Marrow-derived Mesenchymal Stem Cells )
J Biol Chem. 2008 April 4; 283(14): 9136-9145
Link to the Protocol

RNAi by dsRNA soaking
Science 26 September 2003: 1921
Link to the Protocol

RNAi screen in Oct4GiP cells
Genes Dev. 2009 April 1; 23(7): 837-848
Link to the Protocol

Screening for effective siRNA using a reporter system
J Mol Biol. 2007 March 2; 366(4): 1064-1073.
Link to the Protocol

Short RNA cloning and sequence analysis
RNA. 2006 December; 12(12): 2092-2102.
Link to the Protocol

shRNA sequence design and constructs
Blood. 2008 February 1; 111(3): 1182-1192
Link to the Protocol

Silencing of gene products enriched in hEC and hES cells
BMC Dev Biol. 2007; 7: 46.
Link to the Protocol

siRNA
PLoS Genet. 2008 Oct;4(10):e1000241
Link to the Protocol

siRNA -Enhancer Screen
PLoS One. 2009 Oct 5;4(10):e7339
Link to the Protocol

siRNA -Suppressor Screen
PLoS One. 2009 Oct 5;4(10):e7339
Link to the Protocol

siRNA Knockdown of the PBR in HeLa Cells
Mol Pharmacol. 2008 Aug;74(2):443-53.
Link to the Protocol

siRNA transfection (Fetal MSCs)
RNA. 2007 August; 13(8): 1179-1183.
Link to the Protocol

siRNA TransfectionPrimary screen
PLoS One. 2009 Oct 5;4(10):e7339
Link to the Protocol

Small Interfering RNA (siRNA) Experiments ( mouse embryonic stem cells)
BMC Genomics. 2008; 9: 155
Link to the Protocol

Stable Suppression of Gene Expression in Murine Embryonic Stem Cells by RNAi Directed from DNA Vector-Based Short Hairpin RNA
Stem Cells 2004;22:93-99
Link to the Protocol

Stealth RNAi
Stem Cells Vol. 24 No. 6 June 2006, pp. 1467 -1475
Link to the Protocol

Temporal and Spatial Regulation of RNAi in Vivo
Proc Natl Acad Sci U S A. 2008 September 16; 105(37): 13895-13900
Link to the Protocol

The dsRNA- and siRNA-initiated RISC assays
Science 26 September 2003: 1921
Link to the Protocol

Transfecting Stealth" RNAi or siRNA into Human Mesenchymal Stem (MSC) Cells Using Lipofectamine" 2000
Invitrogen
Link to the Protocol

Validation of RNAi Compounds Targeting OCT4 and Nanog
Stem Cells Vol. 24 No. 6 June 2006, pp. 1467 -1475
Link to the Protocol

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