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Methods for detecting a target nucleic acid and a target protein in a single assay [Patent News]
(Posted 03/19/2012)
This US patent application describes a diagnostic method for the detection of a target nucleic acid and a target protein in single assay. In this method, aptamers that bind to target proteins will be added to a mixture of nucleic acid and proteins and the target protein and nucleic acids in the mixture can be simultaneously detected using PCR or similar methods. For example, as reported in this patent application, bladder or colorectal cancer can be detected in complex mixture that contains the genes ( e.g. FGFR3, K-ras, K-ras2, APC, DCC, TP53, PRC1, NUSAPI1, CAPZ, PFKP, EVER1, FLT1, ESPL1, AKAP2, CDC45L, RAMP, SYNGR2, NDRG1, ZNF533 etc) and proteins (e.g. MMP-2, MMP-9, MMP9/NGAL complex, MMP/TIMP complex, MMP/TIMP1 complex, ADAMTS-7, ADAM-12, thymosin 15, thymosin 16, profilin, prohibitin, ubiquitin, tropomyosin, Cyr61, cystatin B, and chaperonin 10).
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Keywords:
Tissue Microarrays, Tissue Arrays,Tissue Arrayer,Tissue Microarray Slides,FFPE tissue, Frozen Human Tissue,Cancer Tissue, research tissue, Monoclonal Antibodies,Antibody Labeling, Antibody Detection,Secondary Antibodies,Primary Antibodies,Antibody Assay,Protein Quantification,Proteomics,protein mass spectrometry,peptide mass spectrometry,peptide fractionation,MALDI-TOF,LC-MS/MS,Quantitative proteomics,Post Translational Modification,phosphoproteins,phosphopeptides,glycopeptide isolation,phosphoprotein isolation,peptide quantitation,trypsin digestion,chymotrypsin,Endoproteinase, Proteomics Software, Proteomics tools,
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