Protein Biomarker Discovery Protocols
Depletion of high abundance proteins from plasma and iTRAQ labeling
In all 3 iTRAQ experiments, the number of plasma samples stated was pooled together according to their classification (i.e. normal, early gastric cancer, late gastric cancer or lung cancer) prior to depletion of high abundance proteins. For the first and second sets of iTRAQ experiment, a total of 35 mg of protein was pooled from each sample. The pooled samples were then delipidated by centrifugation at 130,000 x g at 4°C for 2 hr. The top transparent layer of plasma resulting from ultracentrifugation was collected and the total protein estimated using BCA assay (Pierce Biotechnology, IL, USA). Plasma samples were then subjected to depletion using lgY-12 spin column (Beckman Coulter, CA, USA) according to the manufacturer's protocol .
Disclaimer: Sciclips does not guarantee the authenticity or validity of the protocols listed in this section. These protocols are extracted "as is" from various sources, with minor modifications. Please refer to the respective citations, which are hyperlinked, for more details. The use of cited molecules, not limited to proteins, nucleic acids, compounds etc, in the protocols that are extracted from patents may be covered under patent rights. These molecules should be used for information purpose only.
Search Biomarker Database
This feature is available only to customers who have subscribed to premium membership.
If you have subscribed to our
premium membership please login
to access this feature.
If you wish to signup for Sciclips/subscribe to our
premium membership, please click here
You will need a Sciclips account to subscribe.
|Fostering innovation through scientific intelligence|
Sciclips consultancy service team is comprised of highly qualified and experienced scientists who have several years of research experience in
academic and industrial laboratories. We offer following custom services at competitive price:
Scientific and technical areas covered: Drug discovery, Stem cells, Genomics, Proteomics, Biomarkers, Clinical diagnostics,
Companion diagnostics, Molecular diagnostics, Personalized medicine (theranostics), Plant and agricultural biotechnology
Patent search: Custom intellectual property (IP) analysis; Comprehensive patent database search and evaluation
Custom data analysis and data mining: From patents, research articles, meeting abstracts and web based information. Custom meta analysis.
Competitive intelligence analysis: Comprehensive analysis of competitive technologies and products; Technology trend prediction; Identification of technology licensing opportunities; Acquisition target identification; Identification of collaborative technology development opportunities.
Technology development: Identification of potential development opportunities in drug discovery assay development, cell based assay development, recombinant protein expression (in vitro and in vivo) and mass spectrometry/proteomics reagents and tools; Product development strategy.
Diagnostics and Theranostics technology development: Diagnostic assay development; Diagnostic target identification (molecular or protein or miRNA or metabolomic biomarkers); Prognostic and drug efficacy biomarkers; Theranostics tools, biomarkers and therapeutic drug targets; Identification of emerging technologies and methods.
Custom database development
Customized bioinformatics tools: Offered through our partners.
Proteomics and Drug discovery services (laboratory based): Offered through our partners.
For more information on our consultancy services please contact us at firstname.lastname@example.org
or email us using the contact form
Receive news updates by email
Tissue Microarrays, Tissue Arrays,Tissue Arrayer,Tissue Microarray Slides,FFPE tissue, Frozen Human Tissue,Cancer Tissue, research tissue, Monoclonal Antibodies,Antibody Labeling, Antibody Detection,Secondary Antibodies,Primary Antibodies,Antibody Assay,Protein Quantification,Proteomics,protein mass spectrometry,peptide mass spectrometry,peptide fractionation,MALDI-TOF,LC-MS/MS,Quantitative proteomics,Post Translational Modification,phosphoproteins,phosphopeptides,glycopeptide isolation,phosphoprotein isolation,peptide quantitation,trypsin digestion,chymotrypsin,Endoproteinase, Proteomics Software, Proteomics tools,
mass spectrometry software,MALDI matrix,MALDI sample preparation,peptide analysis,peptide idetification,peptide mapping,peptide characterization,peptide isolation,protein sequencing,peptide sequencing,peptide labeling,peptide modification,custom proteomics, tandem affinity purification, affinity purification, yeast two-hybrid, yeast three-hybrid, protein sequencing,genotyping,next generation sequencing, RNA sequencing, parallel sequencing, genome sequencing,exon sequencing, sequencing assembly, sequencing software, SNP sequencing, genotype sequencing, transcriptome sequencing, Pyrosequencing, exome sequencing, Genome Wide Association