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Bioprotocols
Biomarker Discovery Protocols: This database contains a comprehensive list of various bioprotocols that are used in biomarker discovery. These complete protocols include techniques used in molecular and protein biomarker discovery research. We have listed unique protocols that are not available in any other online bioprotocol database.

Protein Biomarker Discovery Protocols

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Acridinium-linked immunosorbent assay (ALISA) for the Detection of Proteins in Human Sera and Cell Culture

Affinity purification of HLA molecules

Analysis and quantitation of protein bound homocitrulline

Analysis of anti-CD38 antibodies by an enzyme immunoassay

Anti-dsDNA ELISA

Antibody Affinity Calculation (e.g. for CTGF )

Antibody Avidity Calculation (e.g. for CTGF)

Antibody Microarray Preparation and RCA Microarrray Immunoassay

Antibody-sandwich ELISA to detect a protein

Antobody Cross-Reactivity Determination (e.g. CTGF)

Automated Quantitative Analysis (AQUA RTM) to assess the Prognostic Value of Biomarkers

Beta-Amyloid ELISA Protocol

Brain Beta-Amyloid Accumulation Determination by Immunohistochemistry

Cacner Biomarker discovery protocol- Proteomics approach

Cell based assay for the identification of drug efficacy/response biomarkers

Ciphergen ProteinChip SELDI-TOF-MS Analysis

ClinProt MALDI-TOF-MS serum protein profiling

Comparative proteome analysis of disease vs. normal cells using iTRAQ

Construction of tissue microarrays (TMA)

Cytokine Bead Array Analysis

Depletion of high abundance proteins from plasma and iTRAQ labeling

Detecting protein in a human serum sample (ELISA)

Detection of biomarker in human lung cancer tissue using a polyclonal antibody (e.g. Serpin B13 biomarker)

Detection of phosphorylated proteins in paraffin-embedded tissue sections using immunohistochemistry staining (e.g. PTEN phosphorylation detection)

Detection of Protein Oxidation (Protein Carbonyl Assay)

Detection of PSA level in serum using glycopeptide Isolation, heavy-isotope-labeled-peptide standards, and 2D-LC-MALDI-TOF/TOF-MS analysis

Determination of Limit of Detection (LOD values)

Determinination of exosome density

Development and validation of an MRM-MS assay-MRM-MS peptide panel selection

Diagnostics of chronic lymphocytic leukemia (CLL) from mononuclears of peripheral blood by Western Blotting

Direct ELISA Assay Protocol

Edman degradation sequencing of proteins isolated from 2-D gels

ELISA and Indirect Inmunofluorescence for Determining pANCA Status

ELISA for IgA anti-I2 Antibodies

ELISA for the measurement of DPPIV/Seprase in human serum and plasma samples

ELISA formats (One-site, sandwich and amplified sandwich ELISA)

Enzyme-Linked Immunosorbent Assay (ELISA) Procedure

Epitope mapping using bacterial display

Epitope mapping using Luminex

Establishment of a Competitive ELISA (Enzyme Linked Immunosorbent Assay) Protocol for Quantification of Serum Biomarkers

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