L-Proline induces differentiation of ES cells (Posted 06/01/2010) The development of cell therapeutics from embryonic stem (ES) cells will require technologies that direct cell differentiation to specific somatic cell lineages in response to defined factors. The initial step in formation of the somatic lineages from ES cells, differentiation to an intermediate, pluripotent primitive ectoderm-like cell, can be ...
TNFalpha imparts breast cancer cells with stem cell-like features (Posted 06/01/2010) Extracellular and intracellular mediators of inflammation, such as Tumor Necrosis Factor alpha (TNFalpha) and NF-kappaB (NF-kappaB), play major roles in breast cancer pathogenesis, progression and relapse. SLUG, a mediator of the epithelial-mesenchymal transition process, is over-expressed in CD44(+)/CD24(-) tumor initiating breast cancer cells and in basal-like carcinoma,...
Lamina propria of adult human oral mucosa harbors a novel stem cell population (Posted 05/18/2010) The highly regenerative capacity of the human adult oral mucosa suggests the existence of a robust stem cell (SC) population in its lamina propria (OMLP). The purpose of this study was to characterize the availability, growth, immunophenotype, and potency of this presumable SC population. Cells positive for the embryonic stem cell transcription...
Mesenchymal stem cells enhance growth and metastasis of colon cancer (Posted 05/18/2010) Recently, mesenchymal stem cells (MSCs) were reported to migrate to tumor stroma as well as injured tissue. We examined the role of human MSCs in tumor stroma using an orthotopic nude mice model of KM12SM colon cancer. In in vivo experiments, systemically injected MSCs migrated to the stroma of orthotopic colon tumors and metastatic...
STAT3 inhibition in cancer stem cells for the treatment of malignant glioblastoma (Posted 05/18/2010) Glioblastoma (GBM), the highest-grade form of gliomas, is the most frequent and the most aggressive. Recently a subpopulation of cells with stem cells characteristics, commonly named "tumor initiating stem cells" (TISC) or "cancer stem cells" (CSC) were identified in GBM. These cells were shown to be highly resistant to chemotherapeutic ...
Autograftable mesenchymal stem cells expressing cytosine deaminase for the treatment of recurrent glioblastomas (Posted 05/18/2010) Suicide genes have recently emerged as an attractive alternative therapy for the treatment of various types of intractable cancers. The efficacy of suicide gene therapy relies on efficient gene delivery to target tissues and the localized concentration of final gene products. Here, we showed a potential ex-vivo therapy that utilized mesenchymal stem cells...
Zscan4 regulates telomere elongation and genomic stability in ES cells (Posted 04/03/2010) Exceptional genomic stability is one of the hallmarks of mouse embryonic stem (ES) cells. However, the genes contributing to this stability remain obscure. We previously identified Zscan4 as a specific marker for two-cell embryo and ES cells......
Isolation and characterization of hepatic cancer cells with stem-like properties from hepatocellular carcinoma (Posted 04/03/2010) Major burdens in the treatment of hepatocellular carcinoma (HCC) are the high percentage of recurrence and resistance to chemotherapy. Hepatic cancer stem cells provide a reservoir of cells that can self-renew, maintain the tumor by generating differentiated non-stem cells which make up the bulk of the tumor and are responsable for recurrence after ablative surgery .....
Lgr6 Marks Stem Cells in the Hair Follicle That Generate All Cell Lineages of the Skin (Posted 03/19/2010) Mammalian epidermis consists of three self-renewing compartments: the hair follicle, the sebaceous gland, and the interfollicular epidermis. We generated knock-in alleles of murine Lgr6, a close relative of the Lgr5 stem cell gene. Lgr6 was expressed in the earliest embryonic...
The most complete systems biology study of cancer stem cell (CSC) (Posted 03/07/2010) A glioblastoma stem cell (GSC) line, GSC11, grows as neurospheres in serum-free media supplemented with EGF (epidermal growth factor) and bFGF (basic fibroblast growth factor), and if implanted in nude mice brains, will recapitulate high-grade glial tumors....
Pluripotent stem cells are susceptible to NK cells (Posted 02/15/2010) Multipotent adult germ-line stem cells (maGSCs) and induced pluripotent stem cells (iPSCs) could be used to generate autologous cells for therapeutic purposes, which are expected to be tolerated by the recipient. However, effects of the immune system on these cells have not been investigated. We have compared the susceptibility of ...
Alpha-fetoprotein producing cells act as cancer stem cells (Posted 02/15/2010) We aimed to demonstrate that alpha-fetoprotein (AFP)-producing cells in cholangiocarcinomas possessed cancer stem cell (CSC)-like properties. AFP enhancer/promoter-driven EGFP gene was transfected into human cholangiocarcinoma cell lines. One cell line, RBE, expressed both AFP and EGFP. Clonal analyses revealed that one ...
Cancer stem cells isolated from colorectal cancer cell lines (Posted 02/03/2010) Cancer stem cells (CSCs) are the subpopulation of cells within a tumor that can self-renew, differentiate into multiple lineages, and drive tumor growth. Here we describe a two-pronged approach for the identification and characterization of.....
Methods of producing stem cells parthenogenetically by activating human oocytes by oxygen tension (Posted 06/17/2010) Methods of producing human stem cells are disclosed for parthenogenetically activating human oocytes by manipulation of O.sub.2 tension, including manipulation of Ca.sup.2+ under high O.sub.2 tension and contacting oocytes with serine threonine kinase inhibitors under low O.sub.2 tension, isolating inner cell
Preparing cardiomyocytes selectively and efficiently from ES cells using inhibitors of BMP signaling (Posted 06/17/2010) For a method of inducing differentiation of cardiomyocytes from stem cells, a method is provided to induce efficiently and selectively differentiation of cardiomyocytes by such a method in which the stem cells are cultured to induce differentiation into cardiomyocytes in the presence of a substance that
Hematopoietic stem cells for the treatment of infarct area injury following an acute myocardial infarction (Posted 06/17/2010) The described invention provides pharmaceutical compositions for treating an infarct area injury and methods of treating or repairing the infarct area injury in a revascularized subject in the aftermath of an acute myocardial infarction resulting from a natural disease process by administering to the subject parenterally through a catheter a
Production and Use of Rat Spermatogonial Stem Cell Lines (Posted 06/17/2010) A spermatogonial stem cell line that is derived from testes of rats characterized by a desirable genetic background can serve as a source for cells to transplant into male-sterile recipient animals that are immuno-compatible with the spermatogonial line. Rat cells thus transplanted readily develop into fertilization-competent, haploid male gametes
Angiocidin Inhibits melanoma stem cell proliferation (Posted 06/17/2010) Methods are presented for the therapeutic administration of angiocidin in the treatment of cancers such as glioma, breast cancer, and leukemia.
Stem cell therapeutic composition without DMSO (Posted 06/17/2010) The present invention provides a therapeutic composition comprising: (i) Trolox, Na+, K+, Ca2+, Mg2+' Cl-, H2PO4-, HEPES, lactobionate, sucrose, mannitol, glucose, dextran-40, adenosine, glutathione; and (ii) stem cells or progenitor cells
Stem cells for treating treating frontal sinusitis (Posted 06/17/2010) The invention relates to compositions and biological regenerates comprising mesenchymal stem cells and osteoinductive materials, such as bioactive glasses and glass ceramics, for use in frontal sinus obliteration,
Adipose derived stem cells and CD26 antagonist or inhibitor for the treatment of immune disorders (Posted 06/17/2010) The present invention provides a method for the preparation of adipose derived stem cells for use in cellular therapy. In further aspects the present invention provides cells produced by the method of the invention, pharmaceutical compositions and kits suitable for use in treatment of patients
Stem cells from urine (Posted 06/17/2010) Provided herein are stem cells and methods for producing a culture of stem cells from urine. The stem cells may be differentiated into an osteogenic, chondrogenic, adipogenic, endothelial, neurogenic or myogenic lineage
Stem cell mobilization to brain using G-CSF; a method for treating Parkinson's disease (Posted 06/01/2010) The invention relates to the discovery that in an animal model of Parkinson's disease (PD), administration of granulocyte colony-stimulating factor (G-CSF) to rodents having 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD restored the function of dopamine neurons
HES6, CD166, CEACAM6, CD59, and CD49f as markers for the isolation and teatment of colon and head and neck cancer stem cells (Posted 06/01/2010) The present invention relates to compositions and methods for characterizing, treating and diagnosing cancer. In particular, the present invention provides a cancer stem cell profile, as well as novel stem cell cancer markers useful for the diagnosis, characterization, prognosis and treatment of cancer and in particular the targeting of solid tumor stem cells.
Isolation of mouse mammary cells using CD45, Ter119, CD35 and optionally CD140a antibodies (Posted 06/01/2010) The present invention relates to an improved method that permits the differential isolation of mouse mammary stem cells and colony forming cells (CFCs). The method involves depletion of non-epithelial cells from freshly dissociated mouse mammary tissue by incubation with an antibody composition containing antibodies specific for CD45, Ter119, CD35 and optionally CD140a. After formation of conjugates between the non-epithelial mammary cells
Stem cells for the treatment of hearing loss, auditory neuropathy , vestibular dysfunction (Posted 06/01/2010) This disclosure relates to methods and compositions for modulating (e.g., increasing) Atohl activity (e.g., biological activity) and/or expression (e.g., transcription and/or translation) in vivo and/or in vitro, e.g., in a biological cell and/or in a subject.
Selective differentiation of cardiomyocytes from stem cells by inhibiting BMP signaling (Posted 06/01/2010) For a method of inducing differentiation of cardiomyocytes from stem cells, a method is provided to induce efficiently and selectively differentiation of cardiomyocytes by such a method in which the stem cells are cultured to induce differentiation into cardiomyocytes in the presence of a substance that inhibits BMP signaling.
Placental stem cells for the treatment of lung diseases or disorders (Posted 06/01/2010) Provided herein are compositions and methods of treatment of individuals having a disease, disorder or condition of the lung, comprising administering a therapeutically- effective amount of placental cells, e.g., placental stem cells.
